副猪嗜血杆菌高免血清的制备与应用.pdf

中国农学通报 2010,26(13): 14-16 Chinese Agricultural Science Bulletin 副猪嗜血杆菌高免血清的制备与应用 徐引弟，王治方，朱文豪，焦海燕，梁 跃，郭成留 （河南省农业科学院畜牧兽医研究所，郑州450002 ） 摘 要：利用华中农业大学蔡旭旺博士分离鉴定的副猪嗜血杆菌作为标准菌株培养灭活后，2 次免疫家 兔后，耳静脉攻毒，心脏采血，琼扩试验检测抗体效价，抗体在1:8 以上颈静脉放血，分离血清，用于琼扩 试验检测河南省农科院畜牧兽医研究所传染病研究室从河南省发病猪场分离鉴定的副猪嗜血杆菌的血 清型，所分离的33 株菌中有12株4 型，9 株5 型，6 株13 型，3 株14 型，2 株12 型，1株2 型，未分出其他血 清型。结果表明，河南省副猪嗜血杆菌主要以4 型、5 型和13型为主要流行的血清型。 关键词：副猪嗜血杆菌；高免血清；琼扩试验；血清分型 中图分类号：S82 文献标志码：A 论文编号：2009-2804 Preparation and Application of Hyper Immunized Serum of Haemophilus parasuis Xu Yindi, Wang Zhifang, Zhu Wenhao, Jiao Haiyan, Liang Yue, Guo Chengliu (Institute for Animal Husbandry and Veterinary Research, Henan Academy of Agricultural Sciences, Zhengzhou 450002) Abstract: The standard sera Haemophilus parasuis (Hps), which were isolated and identified by Dr. Cai Xue-wang of Huazhong Agricultural University, were cultured, killed and immunized rabbits twice. The rabbits were challenged by ear vein injection and blood from heart. The antibody titers were detected by Gel Diffusion (GD) test. The rabbits were blood by jugular vein if the titers were more than 1:8. The serum were separated and used to detect the serotype of Hps which were isolated from Henan pig farms by the lab of with GD method. There are 12 strains serovar 4, 9 strains serovar 5, 6 strains serovar 13, 3 strains serovar 14, 2 strains serovar 12 and 1 strains serovar 2, and no other serovar among 33 isolates. The results showed that serovar 4, 5, 13 were the major prevalent serotype in Henan. Key words: Hps; hyper immunized serum; GD test; serotype [3] [4] 0 引言