高效液相色谱法测定关黄柏与川黄柏的有效成分的含量分析.pdf

174 eilongjiangM edicine JournalVol24No2 2011 高效液相色谱法测定关黄柏与川黄柏的有效成分的含量分析 , 黑龙江省黑河市药品检验所( 164300) :采用高效液相色谱法: 以十八烷基硅烷键合硅胶为填 剂;以乙腈 - 01%磷酸溶液( 50: 50) (每 100m l加十二烷基磺酸钠 01g)为流动相; 流速 1m l/min; 检测波长 265nm; 理论板数按盐酸小檗碱峰计算不应低于 4000: 川黄柏中所含有抗病原微生物作用的活性成分盐酸小檗碱高于关黄柏中所含盐酸小檗碱, 平均约 3- 4 倍, 川黄柏中不含盐酸巴马 丁, 关黄柏中盐酸巴马 丁含量比较高 :关黄柏; 川黄柏;盐酸小檗碱 : R9272 : A : 1006- 2882( 2011) 02- 174- 02 Determ ination ofCortex Phellodendri Amurensis and Cortex Phellodendri Ch inensis byHPLC Gao F en g, et al eihe In stitute for Drug Control( 164300) Abstract: Objective:The PLC methodwas used: 18 alkyl silane for bonding silicone filler; acetonitrile - 01% phos phoric acid(50: 50) ( per 100ml and 01 g sodium dodecyl) as themobilephase flow rate of 1ml /m in; detectionwavelength was 265 nm; on the number of theoreticalplates berberine should not be lower than the peak of4000Results: Comm issioner uangba,i Sichuan uangbaiberberine reference substance and the corresponding location of the spots, and hydrochloric acid clearance uangbaiPalmatine reference substance corresponding location of the spots, and no- uangbai can accurately iden tify and customs uangbai- uangbai- Resistant uangbai contained in the original role of the m icrobes berberine active ingredients contained in the above related uangbaiberberine, an average of about 3- 4 tmi es K ey words:P hellodendr iA m urensis; Cortex P hellodendr i Ch inensis; berberine ,; , , ,, ,, , , , [ 1] , , 2005 [ 2] 3 1