骨髓间充质干细胞移植治疗老年性痴呆模型大鼠的行为学检测.doc

骨髓间充质干细胞移植治疗老年性痴呆模型大鼠的行为学检测 张 颖，郭建华，闫洪娟，罗秋华，李鑫萍，郭 玮，王海霞(邯郸市第一医院老年病二科，河北省邯郸市 056000) 引用本文：张颖，郭建华，闫洪娟，罗秋华，李鑫萍，郭玮，王海霞. 骨髓间充质干细胞移植治疗老年性痴呆模型大鼠的行为学检测[J].中国组织工程研究，2016，20(36):5371-5377. DOI: 10.3969/j.issn.2095-4344.2016.36.008 ORCID: 0000-0002-2858-3952(张颖) 文章快速阅读： 文题释义： Morris水迷宫β-淀粉样蛋白1-40制作老年性痴呆模型，造模后3周，干细胞治疗组大鼠双侧海马区注射诱导分化后的骨髓间充质干细胞悬液，模型对照组和假手术组不做任何处理。采用Morris水迷宫检测各组实验大鼠学习记忆能力，苏木精-伊红染色观察脑组织病理形态变化。 结果与结论：①造模成功后，模型对照组大鼠的逃避潜伏期高于假手术对照组，跨越平台次数低于假手术对照组；②细胞移植后第1，2，4，8，10周，随着治疗时间的延长，干细胞治疗组大鼠的逃避潜伏期明显缩短，跨越平台次数明显增加。与模型对照组相比，干细胞治疗组大鼠的学习、记忆能力有所改善；③干细胞治疗组脑组织细胞形态比较完整，细胞变性、坏死数量与模型对照组相比明显减少；④结果表明，骨髓间充质干细胞移植能有效改善老年性痴呆大鼠的学习和记忆能力，对老年性痴呆有一定的治疗作用。 关键词： 干细胞；移植；骨髓间充质干细胞；老年性痴呆；学习记忆；大鼠 主题词： 骨髓；间质干细胞移植；阿尔茨海默病；组织工程 基金资助： 河北省医学科学研究重点课题 Bone marrow mesenchymal stem cell transplantation improves behavior performance of senile dementia rats Zhang Ying, Guo Jian-hua, Yan Hong-juan, Luo Qiu-hua, Li Xin-ping, Guo Wei, Wang Hai-xia (Second Department of Geriatrics, First Hospital of Handan, Handan 056000, Hebei Province, China) Abstract BACKGROUND: More recently, studies have demonstrated that bone marrow mesenchymal stem cells can be induced in vitro to differentiate into neuron-like cells that are used for in vivo transplantation to repair nerve damage. OBJECTIVE: To study the effect of bone marrow mesenchymal stem cell transplantation on learning and memory ability of senile dementia rats. METHODS: Thirty male Sprague-Dawley rats were randomly divided into three groups: normal control group, stem cell therapy group and model control group. Rats in the latter two groups were used to establish animal models of senile dementia by intracranial injection of β-amyloid 1-40. Three weeks after modeling, rats were given bilateral hippocampal injection of induced bone marrow mesenchymal stem cell suspension in the stem cell therapy group, whereas no treatment was given in the normal control and model control groups. Morris water maze test was used to detect learni