甘蓝型油菜种质资源的聚类分析.PDF

安徽农业大学学报, 2017, 44(5): 918-923 Journal of Anhui Agricultural University [DOI] 10.13610/ki.1672-352x030 网络出版时间：2017/10/17 11:04:18 [URL] /kcms/detail/34.1162.S1104.060.html 甘蓝型油菜种质资源的聚类分析 唐章林，罗 艺，李阳阳，耿晓宇 (西南大学农学与生物科技学院，重庆 400715) 摘 要：杂交育种是油菜育种的主要方法之一。为研究甘蓝型油菜种质资源的遗传多样性，以提高甘蓝型油菜种 质资源的利用效率，采用近红外光谱法（NIRS ）测试了208 份甘蓝型油菜种质资源的12 个品质性状，方差分析结果 表明各性状均达到极显著差异；通过聚类分析的类平均法在聚类阈值λ=4.86 处将208 份资源划分为6 大类，其中第1 大类包含170 份资源，在聚类阈值λ=3.70 处第 1 大类比较明显的分为4 个亚类；通过聚类分析的离差平方和法在聚 类阈值λ=27.00 处参试资源比较明显的分为7 大类。综合2 种聚类分析结果最终将208 份资源分为13 个类群。 关键词：甘蓝型油菜；种质资源；聚类分析 中图分类号：S565.4 文献标识码：A 文章编号：1672352X (2017)05091806 Clustering analysis of rapeseed (Brassica napus L.) germplasm resources TANG Zhanglin, LUO Yi, LI Yangyang, GENG Xiaoyu (College of Agronomy and Biotechnology, Southwest University, Chongqing 400715) Abstract: Hybridization is one of the main methods for rapeseed breeding in China. In order to study the genetic diversity of rapeseed (Brassica napus L.) germplasm resources to improve their utilization efficiency, 12 quality traits of 208 cultivars were tested by near infrared reflectance spectroscopy (NIRS). The results showed that all the tested traits were significantly different in variance analysis. The 208 cultivars were divided into 6 groups at clustering threshold λ = 4.86 by cluster analysis using unweighted pair-group method with arithmetic means (UPGMA), in which the first group including 170 cultivars was obviously divided into 4 subgroups at clustering threshold λ = 3.7. Using cluster analysis with sum square of deviation method, all the tested cultivars were quite clearly divided into 7 groups at clustering threshold λ = 27.00. Based on the comprehensive analysis of the two c