利用SSR标记定位明恢63的2对恢复基因.pdf

, 29( 9) : 798~ 802, 2002 Acta Genetica Sinica SSR 63 2 1 2 2 1 何光华, 王文明, 刘国庆, 侯 磊, 1 1 1 1, 肖月华, 唐 梅, 杨正林, 裴 炎 ( 1. , 400716; 2. , 100101) : 97A 63 F , 302 SSR 2 , , 1RM1 10 RM258RM304 , F , , 1. 9 2 2. 9 0. 0cM BT 3 10 : ; 63; ; : Q943 : A : 0379- 4172( 2002) 09- 0798- 05 Mapping of Two Fertility-restoring Gene for WA Cytoplasmic Male Sterility in Mingh i63 Using SSR Markers 1 2 2 1 HE Gu ng-Hu , WANG Wen-Ming , LIU Guo-Qing , HOU Lei , 1 1 1 1, XIAO Yue-Hu , TANG Mei , YANG Zheng-Lin , PEI Y n ( 1. Center of Biotechnology, Southwest Agricultural University, Chongqing 400716, China; 2. Institute of Genetics and Develo mental Biology , Chinese A cademy of Sciences, Beij ing 100101, China) Abstract: F2 popul tion derived from Sh nyou63, F1 hybrid developed from the cross Zhensh n97 A/ Zhensh n97B,w s used in this study. Fertile bulkw s constructed by polling equ l mount of 15 highly fertile lines. Sterile bulk w s obt ined by pooling equ l mount of 15 highly sterile lines. Minghui63 nd Zhensh n97A, p rents of Sh nyou63, were n lyzed with 302 p irs of SSR primers. 244 p irs of primers g ve mplific tion products, of which 58 p irs of primers on 12 different chromosomes showed polymorphism between the two p rents with polymorp