乳腺癌VEGF.doc

乳腺癌VEGFC mRNA的表达与淋巴结转移的关系 　　作者：王虎霞，盛 薇，王光辉，樊 林，单 涛，赵 伟，李 萌 作者单位：(西安交通大学医学院第一附属医院普外科，陕西西安 710061) 　　【摘要】 目的 探讨乳腺癌血管内皮生长因子C(VEGFC)的表达与乳腺癌淋巴结转移等临床病理指标之间的关系。方法 用逆转录聚合酶链反应(RTPCR)法检测60例乳腺浸润性导管癌VEGFC mRNA的表达情况，取15例乳腺纤维腺瘤标本作为对照。结果 乳腺癌组VEGFC mRNA的阳性表达率及表达程度明显高于对照组(P均0.01);乳腺癌淋巴结转移组VEGFC mRNA的表达率及表达程度高于淋巴结非转移组(P均0.05);VEGFC mRNA的表达与乳腺癌淋巴结转移呈正相关(r=0.638，P0.01)。结论 乳腺癌中VEGFC mRNA的表达水平增高;VEGFC mRNA的表达促进乳腺癌淋巴结转移。 　　【关键词】 乳腺癌;血管内皮生长因子C mRNA;淋巴结转移 　　Correlation between VEGFC mRNA expression 　　and lymph node metastasis in breast cancerWANG Huxia, SHENG Wei, WANG Guanghui, FAN Lin, SHAN Tao, ZHAO Wei, LI Meng 　　(Department of General Surgery, the First Affiliated Hospital, Medical School of 　　Xian Jiaotong University, Xian 710061, China)ABSTRACT: Objective To study the expression of vascular endothelial growth factor C (VEGFC) in breast cancer and its correlation with clinicopathological indexes of lymph node metastasis. Methods Reverse transcription polymerase chain reaction (RTPCR) method was adopted in detecting VEGFCmRNA expression in 60 cases of breast infiltrative tubular cancer. We took 15 cases of breast fibroadenoma as controls. Results The positive rate and degree of VEGFCmRNA expression were higher in the breast cancer group than in the control group (P0.01). VEGFCmRNA expression was significantly higher in the lymph node metastasispositive group than in lymph node metastasisnegative group (P0.05). VEGFCmRNA expression was positively correlated with lymph node metastasis (r=0.638, P0.01). Conclusion VEGFCmRNA expression was higher in breast cancer and it promoted lymph node metastasis in breast cancer. 　　KEY WORDS: breast cancer; vascular endothelial growth factor C mRNA; lymph node metastasis 　　研究表明，血管内皮细胞生长因子C(vascular endothelial growth factor C, VEGFC)是较特异的促淋巴管内皮生长因子，在乳腺癌淋巴管的生成及淋巴结转移中发挥着不可忽视的作用[1]。目前关于VEGFC基因水平的研究，国内报道较少。本研究通过逆转录聚合酶链反应(reverse transcription polymerase chainreaction, RTPCR)法检测乳腺癌组织中VEGFC mRNA的表达