实验性糖尿病肺形态学及氧自由基研究.doc

???? 实验性糖尿病肺形态学及氧自由基研究 ???? ????［摘　要］　目的：了解早期糖尿病肺形态学及氧化应激的变化。方法：应用体视学方法对四氧嘧啶诱导的糖尿病28 d大鼠肺进行定量研究、观察超微结构变化并测定血清和肺组织的超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量。结果：糖尿病大鼠肺泡体密度和平均截线长度减少，肺泡壁体密度、肺泡表面积密度、肺泡数密度、肺泡面数密度和肺泡比表面增加。糖尿病大鼠Ⅱ型肺泡细胞粗面内质网扩张，其体密度、面密度、平均截面积和平均周长增加，而比表面减少。肺上皮和毛细血管基底膜增厚。糖尿病大鼠血清SOD活性下降，MDA含量增加；肺组织MDA含量明显升高。结论:糖尿病早期大鼠肺已发生形态学变化且受到氧化应激的危害，提示肺脏是糖尿病损害的“靶器官”之一。　　［主题词］　　糖尿病，实验性； 肺；超氧化物歧化酶；丙二醛　　［中分类号］　R563.1； R587.1　　　［文献标识码］　A　　［文章编号］　1000-4718(2000)06-0552-05 Study of lung morphologic features and oxygen free radicals in experimental diabetic rats SHEN Xing-ping, SHU Chang-da, ZHANG Zuo-cai, HE Jun(Department of Endocrinology, The Second College of Clinical Medicine,Chongqing University of Medical Sciences, Chongqing 400010, China) 　　［Abstract］　AIM:To evaluate the changes of lung morphologic features and oxidative stress in experimental diabetic rats. METHODS: The pulmonary structure of alloxan-induced diabetic rats were quantitatively studied with stereological methods. Changes of ultrastructure, activities of superoxide dismutase (SOD) and contents of malondialdehyde(MDA) of diabetic lung and serum were observed. RESULTS: The volume proportion of alveolar air and mean linear intercept of diabetic rats decreased remarkably in comparison with controls while the volume proportion of alveolar wall, the surface density of alveolar, the numerical density of alveolic area, the numerical density of alveolar and specific surface of alveolar increased significantly. The major change of the type II pneumocyte of diabetic rats was dilation of the rough endoplasmic reticulum (RER). The other findings in diabetic rats had included the presence of thickened alveolar epithelial, pulmonary capillary basal laminae and blood- air barrier, the volume density, the surface density, the mean profile area and the mean perimeter of RER in type Ⅱ pneumocyte of diabetic rats increased remarkably. And the specific surface of RER was significantly lower as compared with controls. SOD activity decreased