小球藻高密度培养及油脂提取条件的优化.pdf

小球藻高密度培养及油脂提取条件的优化 ＊ 李金穗 汪苹 董黎明 (北京工商大学食品学院, 北京 100048) 摘 要: 【目的】高密度培养小球藻及优化油脂提取条件。【方法】通过进行单因素实验研究不同培养基组成及环 境因子对其细胞生长影响,并采用超声波提取法进行正交实验对藻粉油脂提取条件进行研究。【结果】对椭圆小球 藻Y4 进行异养培养, 最适培养条件为: 葡萄糖50 g /L, 硝酸钾2 g/L, 适宜的培养温度、摇床转速和接种量分别为 29 °C、180 r/min 和20%。在此基础上, 进行了 1 L 发酵罐培养实验, 获得了干重 18.25 g/L 的生物量。通过对油 脂提取条件进行优化, Y4 的油脂提取率由优化前的25.0%提高到60.2%, 提高了35.2%。。【结论】该研究优化了小 球藻的培养条件及油脂提取条件,促进了小球藻的开发和利用。 关键词: 椭圆小球藻, 高密度培养, 油脂提取 Chlorella high density training and lipid extraction condition optimizing LI Jin-sui WANG Ping＊ DONG Li-ming (College of Food Science, Beijing Technology and Business University , Beijing 100048, China) Abstract: [Objective]Chlorella high density training and lipid extraction condition optimizing 。 [Methods] Single factor experiments were used to research different mediums and environmental factors on Chlorella cell growth effects, and the ultrasonic extraction method was employed by orthogonal experiment for algae powder oil extraction conditions. [Results] The optimal condition of Chlorella ellipsoidea Y4 under heterotrophic culture to get high biomass were: BG 11 medium with 50 g/L of glucose as the carbon source and 2 g/L KNO3 as the nitrogen source. The optimum culture temperature, shaking rate and the inoculums size were 29 °C, 180 r/min and 20%. By inoculating the preculture to a fermentation tank of 1 L capacity, we got dry cell weight 18.25 g/L. Through to the oil extraction conditions were optimized, Y4 lipid extraction yield increased from 25% to 60.2%, with lipid extraction yield raised by 35.2%. [Conclusion] Optimization of chlorella culture conditions and lipid extraction conditions were stud