急性髓系白血病CTNNA1基因的表达及临床意义 .pdf

急性髓系白血病CTNNA1基因的表达及临床意义

陈星星;钱军;林江;安翠;马吉春;邓兆群;陈芹;杨静;李云

【摘要】目的:研究急性髓系白血病(acutemyeloidleukemia,AML)患者钙黏蛋

白相关蛋白(α-catenin,CTNNA1)基因的表达情况并探讨其临床意义.方法:应用实

时定量PCR(qRT-PCR)法检测25例非恶性血液病患者(对照组)和92例初诊AML

患者(观察组)中CTNNA1基因的表达水平,并分析其与临床参数的相关性.结果:观

察组中51例(51/92,55.43％)存在CTNNA1低表达,而对照组未发现CTNNA1低

表达(0.00％),两组比较,差异有统计学意义(x2=17.152,P＜0.001);CTNNA1表达水

平与AML患者的年龄、性别、外周血白细胞计数、血小板计数、血红蛋白含量、

FAB分型、核型分组之间均无相关性(P均＞0.05).ROC曲线分析表明CTNNA1表

达水平能有效鉴别观察组和对照组,AUC=0.806.观察组中CTNNA1低表达的患者

化疗后完全缓解率高于对照组,但差异无统计学意义(x2=2.476,P=0.142).4例初诊

AML患者在获得完全缓解后CTNNA1表达水平均较治疗前有所升高.结

论:CTNNA1基因低表达可能是AML中的一个常见分子事件,检测其表达可用于

AML患者的辅助诊断和疾病状态监测.%Objective:Toinvestigatethe

expressionofthehumancateninalpha1(CTNNA1)geneinacutemyeloid

leukemia(AML)anditsclinicalsignificances.Methods:Theexpressionof

CTNNA1transcriptinbonemarrowmononuclearcellsfrom25controls

(controlgroup)and92newlydiagnosedAMLpatients(observationgroup)

weredetectedbyquantitativereal-timepolymerasechainreaction(qRT-

PCR);andtherelationshipbetweenCTNNA1expressionandclinical

parameterswereanalyzed.Results:DownregulatedCTNNA1expressionwas

foundin51AMLcases(55.43％),significantlylowerthanthatinall

25controls(P＜0.001).TherewasnocorrelationbetweenTheCTNNA1

expressionlevelandthesex,age,bloodparametersandFABsubtypes(both

P＞0.05).ROCcurveanalysisrevealedthattheCTNNA1expressionlevel

coulddiscriminateAMLpatientsfromcontrolseffectively(AUC

=0.806).Therewasnostatisticaldifferenceinthecompleteremissionrate

betweencontrolgroupandobservationgroup,thoughthepatientswith

lowCTNNA1expressioninobservationgrouphadhighercomplete

remissionrateafterchemotherapy(x2=2.476,P=0.142).TheCTNNA1

expressionlevelsoffourpa