N_糖基化位点鉴定方法和非经典N_糖基化序列.pdf

第23卷第6期 生命科学 Vol. 23, No. 6 2011年6月 Chinese Bulletin of Life Sciences Jun., 2011 文章编号：1004-0374(2011)06-0605-07 N- N- 糖基化位点鉴定方法和非经典 糖基化序列 周　蕾，顾建新* (复旦大学上海医学院，卫生部糖复合物重点实验室，上海200032) N- 摘　要：蛋白质的 糖基化修饰是生物体调控蛋白质在组织和细胞中的定位、功能、活性、寿命和多样性 N- 的一种普遍的翻译后方式。 糖基化位点是理解糖链功能的重要前提之一。应用新的糖蛋白、糖肽富集技 N- N- 术和质谱技术，科学家们在不同组织中完成了对 糖基化位点的鉴定。此外，不同于经典三联子的 糖 N- 基化序列的发现使人们对 糖基化过程的认识向纵深发展。 N- 关键词： 糖基化；糖基化位点；非经典序列 中图分类号：Q513.3 文献标志码 ：A N-glycosylation sites analysis and nonconsensus N-glycosylated sequences ZHOU Lei, GU Jian-Xin* (Key Laboratory of Glycoconjugate Research, Ministry of Health; Shanghai Medical College, Fudan university, Shanghai 200032, China) Abstract: N-glycosylation of proteins is the most common post-tranlational modification, strongly influencing many of their location, function, activity, life span and diversity. Our knowledge to N-glycosylation sites is one of the most important prerequisites for detailed functional understanding. Using new methods based on glycoproteins/ glycopeptides enrichment and mass spectrum techniques, scientists have been able to identify glycosylated sites in different tissues. Besides, the discovery of novel sequence patterns for N-glycosylation will help to understand the process of N-glycosylation better in future. Key words: N-glycosylation; glycosylation sites; nonconsensus sequences 糖基化(Glycosylation) 是指蛋白质或脂质在酶 差异，糖链分为以下几种类型：(1) 连接于天冬酰 的作用下被连接上糖链的过程。作为生物体内最为