一株拮抗真菌的蛇足石杉内生细菌分离鉴定及培养条件优化-生物谷.doc

一株拮抗蛇足石杉内生细菌分离鉴定曾庆桂 朱笃*朱飞 王志兴江西师范大学生命科学学院南昌330022) 摘 要: 从蛇足石杉Huperzia serrata)叶片中分离到一株对小麦赤霉等多种植物病原真菌有强拮抗作用内生细菌H-6。通过形态和培养特观察、生理生化实验及16S rDNA序列分析, 初步鉴定该菌属伯克霍尔德属, 命名为Burkholderia H-6。同时还对该菌条件进行了优化, 得出马铃薯浸出液中添加2.5%的甘露醇和0.1% NaNO3, 最适培养温度为28, 最佳初始pH4.0。蛇足石杉 内生细菌 拮抗Isolation and Identification of Endophytic Bacteria H-6 with High Antifungal Activity from Huperzia serrata ZENG Qing-Gui ZHU Du* YAN Ri-Ming ZHU Fei WANG Zhi-Xing (College of Life Sciences, Jiangxi Normal University, Jiangxi Provincial Key Lab of Protection and Utilization of Subtropic Plant Resources, Nanchang 330022) Abstract: In this study, nearly 200 endophytic bacteria were isolated from different part of Huperzia serrata, over 60 bacterium with clear antifungal activity were selected from those cultures. Among them, strain H-6 exhibited the highest antifungal activity which was strongly inhibits the growth of many plant pathogenic fungi such as Sclerotinia scleroliorum, Fusarium graminearumt, Sclerotinia libertiana, Phytophthora capsici Leonia and Sesame fusarium wilt. According to the characteristics of morphology, physiology and biochemistry tests and the comparison of 16S rDNA sequence, the strain H-6 was similar to the Burkholderia. So strain H-6 was identified as Burkholderia sp. H-6. The results also showed that Burkholderia sp. H-6 was markedly different from Burkholderia cepacia that was applied widely in agriculture as antagonistic bacteria. The medium and culture conditions of the strain all were optimized by single factor and orthogonal experiments. In the investigation of the culture condition, growth was carried out in a basal medium (potato juice) and gradually supplemented with the various ingredients to be investigated. The major ingredients being investigated included carbon sources and nitrogen sources. The optimal antifungal activity production condition is growth in a medium (potato juice with 2.5% mannitol and 0.1% NaNO3), initial pH 4.0 at 28℃. Keywords: Huperzia ser