elisa试剂盒,小鼠肝脂酶(hl)elisa试剂盒.doc

elisa试剂盒，小鼠肝脂酶(HL)ELISA试剂盒 elisa试剂盒，小鼠肝脂酶(HL)ELISA试剂盒 产品规格：96T/48T。 用途：科研实验等领域科学研究，不得用于临床诊断。 Elisa试剂盒说明书，elisa试剂盒价格，ELISA检测试剂盒 elisa试剂盒，小鼠肝脂酶(HL)ELISA试剂盒Kit: Incubation with closure plate membrane sealing plate rear 37 DEG C was incubated for 30 min. With liquid: 30 (20 times 48T) times concentrated washing liquid with distilled water 30 times (20 times 48T) dilution. Washing: be careful torn off the seal plate membrane, discard liquid, drying, washing liquid to fill each hole, standing for 30 seconds after the discard, repeat 5 times, pat dry. Enzyme: per hole adding enzyme reagent 50 L, except the blank hole. The collection and storage of Sample Kit The use of serum serum separating tube, the sample in room temperature for two hours or overnight. At about 1000 * 4oC G. serum centrifugal freshly prepared 20 minutes Storage of samples at -20 DEG C or use 80oC or immediately after dilution. To avoid repeated freeze-thaw cycles. Plasma plasma collection with EDTA or heparin as an anticoagulant. For 15 minutes and centrifuged samples * G in 2 to 8 degrees Celsius within 30 minutes of collection. The removal of plasma and assay immediately or store samples Liquid samples: Including serum, plasma, urine, pleural effusion, cerebrospinal fluid, cell culture supernatant. Serum): Coagulation at room temperature for 10-20 minutes, and centrifuged for 20 minutes or so (2000-3000 rpm). Remove supernatant. If precipitation in the preservation process, centrifugal again. Plasma): According to the requirements of the specimens of choice of EDTA, sodium citrate or heparin as an anticoagulant, 10-20 minutes after mixing, centrifuged for 20 minutes or so (2000-3000 r.p.m.). Remove supernatant. If precipitation in the preservation process, centrifugal again. Urine): A sterile collection. Centrifugal 20 minutes (2000-3000 rpm). Remove supernatant. If precipitation in the preservation process, centrifugal again. Hydrothorax and cerebrospinal fluid with reference to this practice. The su