DB51_T2905-2022_猪A型塞内卡病毒分离鉴定技术规范_四川省.docx

ICS11.220

CCSB41

DB51

四川省地方标准

DB51/T2905—2022

猪A型塞内卡病毒分离鉴定技术规范

TechnicalspecificationforisolationandidentificationforswinesenecavirusA

四川省市场监督管理局

发布

DB51/T2905—2022

目

次

前言.................................................................................II

1

2

范围...............................................................................1

规范性引用文件.....................................................................1

3术语和定义.........................................................................1

4

5

缩略语.............................................................................1

试剂与设备.........................................................................1

5.1主要试剂.......................................................................1

5.2主要仪器设备...................................................................2

6样品的采集、保存与运输.............................................................2

6.1样品采集.......................................................................2

6.2保存与运输.....................................................................2

7病毒的细胞分离培养.................................................................2

7.1生物安全措施...................................................................2

7.2样品的处理.....................................................................2

7.3单层细胞制备...................................................................2

7.4样品接种.......................................................................3

7.5病毒收获.......................................................................3

8

病毒核酸鉴定.......................................................................3

8.1RT-LAMP和RT-PCR引物...........................................................3

8.2核酸提取和反转录...............................................................3

8.3RT-LAMP反应....................................................................3

8.4RT-PCR反应.........................................