Elabscience One-component TMB Substrate使用说明书用户手册.pdf

7thEdition,revisedinApril,2017

（FORRESEARCHUSEONLY.DONOTUSEITINCLINICALDIAGNOSIS!）

One-componentTMBSubstrate

CatalogNo:E-IR-R201

30mL/100mL/500mL/1000mL

Thismanualmustbereadattentivelyandcompletelybeforeusingthisproduct.

Ifyouhaveanyproblems,pleasecontactourTechnicalServiceCenterforhelp.

Phone:240-252-7368(USA)240-252-7376(USA)

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Copyright©2017-2018ElabscienceBiotechnologyInc.AllRightsReserved

7thEdition,revisedinApril,2017

Description

TMBisapopularchromogenicsubstrateforHRPdetectioninELISAandisavailableinseveralformats.

ThesolubleOne-componentTMBSubstratearesuppliedasReady-to-Usemono-componentliquidsthat

requirenopreparationbeforeuse.Theproductmainlycontains3,3’,5,5’-tetramethylbenzidineand

containsnoDMForDMSO.

One-componentTMBSubstrateyieldsabluecolor(Amax=370nmand652nm)whenreactedwith

peroxidaseinmicrowellapplications.Theproductchangestoyellow(Amax=450nm)uponadditionof

asulfuricorphosphoricacidstopsolution.

Application

Peroxidase-basedenzymeimmunoassaysELISAisrecommended.

Storage

Thisproductisstablefor1yearsstoredat2°C~8°C.Shadinglight.

Productfeatures

Economical:Costlesscomparedwithsimilarproducts.

Convenience:mono-component,ready-to-use.

Highsensitivity:Savethedosageofantibody.

Strongstability:Validfor1yearsat2~8℃.

Stablesignal:Thesignaliscontinuoussteadyafteraddingstopsolution.

Safe:Noncarcinogenic.

Assayprocedure

1.Add100μLoftheTMBSubstrateSolutiontoeachmicroplatewell.

2.Incubatefor5~30minaccordingtothereactionsystem.

3.Stopreactionbyadding50uLstopsolution(1MHClor1MHSO).