基于GEO数据库探讨UBE2T在肺腺癌中的作用.docx

基于GEO数据库探讨UBE2T在肺腺癌中的作用

李晶汪巍彭敦钰

[关键词]?肺腺癌；单细胞分析；TCGA数据库

[中图分类号]?R734??????[文献标识码]?A????[DOI]?10.3969/j.issn.1673-9701.2024.08.005

Investigating?the?role?of?UBE2T?in?lung?adenocarcinoma?based?on?GEO?database

LI?Jing1，?WANG?Wei2，?PENG?Dunyu1

1.Department?of?Anesthesia，?Peoples?Hospital?of?Wuhan?University，?Wuhan?430060，?Hubei，?China;?2.?Department?of?Thoracic?Surgery，?Peoples?Hospital?of?Wuhan?University，?Wuhan?430060，?Hubei，?China

[Abstract]?Objective?To?investigate?the?role?of?ubiquitin?binding?enzyme?EZT?（UBE2T）?in?lung?adenocarcinoma?by?integrating?single-cell?sequencing?data?with?the?TCGA?database，?in?order?to?provide?insights?into?the?specific?molecular?mechanisms?of?UBE2T?in?lung?adenocarcinoma.?Methods?Single-cell?sequencing?data?was?downloaded?from?the?GEO?database?（GSE117570），?and?R?language?was?used?for?quality?control?and?analysis?of?the?data.?Additionally，?online?tools?were?employed?to?analyze?lung?adenocarcinoma-related?data?from?the?TCGA?database.?The?potential?target?genes?associated?with?UBE2T?were?identified?by?integrating?TCGA?and?single-cell?sequencing?data.?Results?Differential?analysis?using?the?TCGA?database?successfully?demonstrated?that?UBE2T?could?serve?as?an?independent?prognostic?factor?in?lung?adenocarcinoma，?and?it?was?correlated?with?poor?patient?outcomes.?Integration?of?single-cell?sequencing?data?revealed?that?UBE2T-associated?genes?were?mainly?distributed?in?mononuclear?cells?and?T?cells.?Furthermore，?analysis?using?the?CancerSEA?database?suggested?a?close?association?between?UBE2T?and?cell?cycle?regulation.?Conclusion?UBE2T?may?play?a?role?in?promoting?the?occurrence?and?development?of?lung?adenocarcinoma?through?the?regulation?of?the?cell?cycle.

[Key?words]?Lung?adenocarcinoma;?Single-cell?analysis;?TCGA?database

泛素连接酶E2T（ubiquitin?binding?enzyme?EZT，?UBE2T）属于UBE2超家族，在泛素化的第二步中起基础作用。以往研究表明UBE2T参与范可尼贫血（Fanconi?anemia，?FA）DNA修复的调控，最近的研究发现UBE2T还参与人类癌症患者的肿瘤发生、转移过程并影响生存和预后[3-4]。癌症组织样本和细胞系中常见到异常的UBE2T表达[5]。相关实验研究证实，UBE2T过表达可促进细胞增殖，而UBE