医学模式图素材.ppt
医学模式图素材
AntibodyToolkit(IgG)
αhelixVimentinActinandmyosinCoiled-coilMicrotubule6nm10nm25nm
Nucleosomes2
ACACDDR1DDR1DDR176123454356217CD45PTP
Metabolismof5-fluorouracil(5-FU)5-FU
Carac,Efudex5-Fluorouridine5-FUMP5-FdUMPUridine
phosphorylase5-FluorodeoxyuridineThymidine
phosphorylaseOrotate
phosphoribosyl
transferaseThymidine
kinase5-FUTP5-FdUTPRNADNA5’-nucleotidase5’-nucleotidaseUridine
kinaseATPADPATPADPPRPP
WesternBlotting1.Resolveproteinsamples
onSDS.Incubatetheblotwith
chemiluminescentHRP
substrateandexposetofilm2.Electrophoreticallytransfer
fractionatedproteinsfrom
gelontoPVDFmembrane3.Blockthemembrane
withneutralprotein
(BSAormilkcasein)4.Incubatethemembrane
withprimaryantibody
specifictotargetprotein5.Incubatethemembrane
withHRP-labeledsecondary
antibodyspecifictoprimary
antibody