类芽孢杆菌BD3526发酵上清对植物乳杆菌ST-Ⅲ的促生长研究.pdf

J『2016,V01．39No．6 乳业科学与技7tt ※基础研究 ST．Ill的促生长研究 洪青，杭锋水，吴正钧 (光明乳业股份有限公司乳业研究院，上海乳业生物工程技术研究中心，乳业生物技术国家重点实验室，上海 200436) 摘要：研究类芽孢杆菌BD3526脱脂乳发酵上清对植物乳杆菌ST．III在脱脂乳中的生长影响。结果表明：培 养5～9d的BD3526脱脂乳发酵上清液可显著促进植物乳杆菌ST—III在脱脂乳中的生长和凝乳。与空白对照相 比，添加含5～9 d发酵上清的脱脂乳培养24 h发生凝乳，活菌数达到 h后，pH值从6．44降至4．70～5．08，16 9．22～9．35(19(CFU／mL))。 关键词：发酵上清；蛋白水解；植物乳杆菌sT．Ⅲ GrowthPromotionofLactobacillusST—HItheFermented ofPaenibacillus plantarumby Supernatant spp．BD3526 HONG Qing，HANGFeng木，WUZhengjun (State of ResearchCenterof KeyLaboratoryDairyBiotechnology，ShanghaiEngineering DairyBiotechnology， Food Research and Dairy Institute，BrightDairy Co．Ltd．，Shanghai200436，China) Abstract：The effectofthe offermentedskimmilkwithPaenibacillus on growth supernatant promoting spp．BD3526 Lactobacillus inoculatedintoskimmilkwas inthis resultsshowedthatthe ST-．III plantarum analyzedstudy．The supernatant ofBD3526culturedins妇nIIliu(for5-9d the ofST．IIIinskim111ilkandexhibitedIllik significantlypromotedgrowth high wasculturedinskinl111ill(addedwiththe ofBD3526for24 valuedecreased ST-111 h．the clottingactivity．After supematant pH to at from6．44 4．7m_5．08．and Wasobserved16h withaviablecellcount miu(clotting along of9．22-_9．35(19(CFU／mL))．