N掺杂的生物基荧光碳点的制备及其检测性能研究.docx

N掺杂的生物基荧光碳点的制备及其检测性能研究

目录

一、内容概要................................................2

1.1研究背景.............................................3

1.2研究意义.............................................3

1.3研究目的与内容.......................................5

二、实验材料与方法..........................................6

2.1实验原料.............................................7

2.2制备方法.............................................8

2.2.1化学氧化法.......................................8

2.2.2模板法...........................................9

2.3检测方法............................................10

2.3.1荧光光谱法......................................11

2.3.2表面增强拉曼光谱法..............................12

2.3.3光致发光光谱法..................................13

三、N掺杂生物基荧光碳点的制备与表征........................14

3.1制备过程............................................15

3.2表征结果............................................16

四、N掺杂生物基荧光碳点的性能研究..........................18

4.1荧光性质............................................19

4.1.1荧光强度........................................20

4.1.2荧光波长........................................20

4.1.3荧光量子产率....................................21

4.2拉曼特性............................................22

4.2.1拉曼散射峰......................................23

4.2.2拉曼活性模式....................................24

4.3光致发光性能........................................26

4.3.1发光强度........................................27

4.3.2发光波长........................................28

4.3.3发光机制........................................28

五、N掺杂生物基荧光碳点在生物传感中的应用..................29

5.1检测生物分子........................................30

5.2检测环境污染物质....................................32

5.2.1检测亚硝酸盐....................................33

5.2.2检测重金属离子..................................33

5.3检测生物病原体......................................35

5.3.1检测大肠杆菌....................................35

5.3.2检测结核杆菌....................................37

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