重组人促红细胞生成素对脓毒症大鼠肺脏的保护作用[权威资料].doc

重组人促红细胞生成素对脓毒症大鼠肺脏的保护作用 本文档格式为WORD,若不是word文档,则说明不是原文档。 最新最全的 学术论文 期刊文献 年终总结 年终报告 工作总结 个人总结 述职报告 实习报告 单位总结 　　【摘要】目的 探讨重组人促红细胞生成素（recombinant human erythropoietin，rHuEPO）对内毒素所致大鼠脓毒症急性肺损伤的保护作用。方法 45只雄性SD大鼠随机（随机数字法）分为对照组、模型组、rHuEPO组，每组均15只。经静脉推注脂多糖LPS（6 mg/kg） 复制急性肺损伤（ALI）的动物模型， rHuEPO组造模前60 min静脉推注rHuEPO（5000 U/kg），观察12 h后处死。留取颈动脉血及肺组织标本。测定氧和指数（OI）、动脉血氧分压（PaO显著降低，差异具有统计学意义（P、IL-6、iNOS水平进而调节炎症反应，对脓毒症所致急性肺损伤具有一定的保护作用。 　　【关键词】重组人促红细胞生成素；脂多糖；脓毒症；急性肺损伤 　　Protective effects of recombinant human erythropoietin on acute lung injury induced by sepsis in rats SANG Zhen-zhen， XU Yun， SHENG Ying-jie， ZHAO Min.Department of Emergency Medicine， Shenjing Hospital ofChina Medical University， Shenyang110004， China 　　Corresponding author： ZHAO Min， Email： zhaom@sj-hospital.org 　　【Abstract】Objective To investigate the protective effects of recombinant human erythropoietin （rHuEPO） on acute lung injury （ALI） induced by lipopolysaccharide （LPS）.Methods Fourty-five rats were randomly （random number） assigned to three groups， namely control group， model group， andrHuEPO group. ALI was induced by intravenous injection of LPS （6 mg/kg）. The rHuEPO （5000U/kg） was injected intravenously into rats 60 min before LPS challenge. The general status of rats was observed. Twelve hours after modeling， the rats were sacrificed and the tissue samples including lung tissue and blood were collected. PaO）］， and inducible nitric oxide synthase （iNOS） were detected. Cytokines were assayed with ELISA method. Pathological changes of lung tissues were observed under light microscope and transmission electron microscopy. Results （1） Compared with the control group， PaO were significantly lower （P， IL-6 and iNOS in serum of rats in the control group were lower than those in the model group and the rHuEPO group （P， IL-6 and iNOS of rats in the rHuEPO group were significantly lower compared with the model group （P0.01）. （4） The light microscopy and the transmission electron microscopy sho