Rho_Rho激酶信号通路在低氧致肺纤维化中的作用与法舒地尔的干预效应.pdf

32 22 V o.l 32, No. 22 2378 2010 11 30 ACTA ACADEM IAE MEDICINAE M ILITARIS TERTIAE Nov. 30 2010 文章编号: 1000-5404( 2010) 22-2378-05 Rho /Rho 1 1 2 1 2 高 艳 , 谢 敏 , 石俊青 ( 61004 1 , ; 610041 , ) [ ] 目的 24 h ( 612 25 mol/L) 方法 ( MRC-5) , H IF-1 , ( 37 5% CO 2% O 3% N ) 061224 h, W estern b lot RhoAROCK 1 2 2 2 CTGF 24 h , 24 h ( connective tissue grow th factor, CTGF) ; RT-PCRMTT 24 h ( MRC-5) CTGF mRNA , 24 h( 21% O , 74% N , 5% CO ) 24 h 2 2 2 24 h, ELISA 24 h( Col ) 结果 , RhoAROCK 1CTGF 24 h ( 61225 mol/L) CTGF mRNA(P 005,P 001) 24 h, 24 h ( 61225m ol/L) Col(P 001)结论 Rho/Rho CTGF, Rho/Rho [] ; ; ; ROCK 1 [] R364. 4; R563. 02; R 78. 2 [] A Fasudil attenuates hypoxia-induced pulm onary fibrosis via suppressing Rho / Rock signal pathway 1 1 2 1 2 Gao Yan , X ieM in , Shi Junqing ( Departm en t of Respiratory D iseases, W est Ch inaH osp ita,l Sichuan University; Departm en t of Resp iratory D iseases, F irst People s H osp ital of Chengdu, Chengdu, Sichuan Province, 610041, Ch ina) [Abstract] O bjective To investigate the effect of hypoxic condition on the expressions of RhoA, ROCK 1and connective tissue grow th factor ( CTGF ) in human fetal fibroblasts, and the beneficial effects of fasud