components of coated vesicles and nuclear pore complexes share a common molecular architecture组件涂层囊泡和核孔复合物共享一个共同的分子结构.pdf

Open access, freely available online PLoS BIOLOGY Components of Coated Vesicles and Nuclear Pore Complexes Share a Common Molecular Architecture 1 2 1 2 3 1* Damien Devos , Svetlana Dokudovskaya , Frank Alber , Rosemary Williams , Brian T. Chait , Andrej Sali , 2* Michael P. Rout 1 Departments of Biopharmaceutical Sciences and Pharmaceutical Chemistry and California Institute for Quantitative Biomedical Research, University of California, San Francisco, California, United States of America, 2 Laboratory of Cellular and Structural Biology, Rockefeller University, New York, New York, United States of America, 3 Laboratory of Mass Spectrometry and Gaseous Ion Chemistry, Rockefeller University, New York, New York, United States of America Numerous features distinguish prokaryotes from eukaryotes, chief among which are the distinctive internal membrane systems of eukaryotic cells. These membrane systems form elaborate compartments and vesicular trafficking pathways, and sequester the chromatin within the nuclear envelope. The nuclear pore complex is the portal that specifically mediates macromolecular trafficking across the nuclear envelope. Although it is generally understood that these internal membrane systems evolved from specialized invaginations of the prokaryotic plasma membrane, it is not clear how the nuclear pore complex could have evolved from organisms with no analogous transport system. Here we use computational and biochemical methods to perform a structural analysis of the seven proteins comprising the yNup84/vNup107–160 subcomplex, a core building block of the nuclear pore compl