combined extraction processes of lipid from chlorella vulgaris microalgae microwave prior to supercritical carbon dioxide extraction结合萃取过程的脂质小球藻寻常的超临界二氧化碳萃取前微藻微波.pdf

Int. J. Mol. Sci. 2011, 12, 9332-9341; doi:10.3390/ijm OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article Combined Extraction Processes of Lipid from Chlorella vulgaris Microalgae: Microwave Prior to Supercritical Carbon Dioxide Extraction Céline Dejoye 1, Maryline Abert Vian 1, Guy Lumia 2, Christian Bouscarle 2, Frederic Charton 2 and Farid Chemat 1,* 1 University of Avignon and Countries of Vaucluse, INRA, UMR408, F-84000 Avignon, France; E-Mails: celine.dejoye@univ-avignon.fr (C.D.); maryline.vian@univ-avignon.fr (M.A.V.) 2 CEA DEN Marcoule, Laboratory of Supercritical fluids and membranes, F-30207 Bagnols sur Cèze, France; E-Mails: guy.lumia@cea.fr (G.L.); christian.bouscarle@cea.fr (C.B.); frederic.charton@cea.fr (F.C.) * Author to whom correspondence should be addressed; E-Mail: farid.chemat@univ-avignon.fr; Tel.: +33-0-490144465; Fax: +33-0-490144441. Received: 1 September 2011; in revised form: 21 October 2011 / Accepted: 2 December 2011 / Published: 13 December 2011 Abstract: Extraction yields and fatty acid profiles from freeze-dried Chlorella vulgaris by microwave pretreatment followed by supercritical carbon dioxide (MW-SCCO ) extraction 2 were compared with those obtained by supercritical carbon dioxide extraction alone (SCCO ). Work performed with pressure range of 20–28