秀丽隐杆线虫的培养与保存研究_李有全.pdf

中国兽医科学　20 11,41(10):100 1-1004 Chinese Ve te rinary Science 中图分类号:S 852.73 1　文献标志码:A 　文章编号:167 3-4 696 (2011)10-1001-04 秀丽隐杆线虫的培养与保存研究 ＊ ＊ 李有全,关贵全, 欲率,何海宁,罗建勋 ,殷　宏 (中国农业科学院兰州兽医研究所 家畜疫病病原生物学国家重点实验室甘肃省动物寄生虫病 重点实验室,甘肃 兰州　730046 ) 　　摘要:比较了在OP50-N G M 、JM 109-NG M 、OP50-LB 、JM 109-LB 四种不同培养基上秀丽隐杆线虫的生 长效果;测定了在三种温度和含不同甘油浓度冻存液中的虫体保存效果。结果显示,秀丽隐杆线虫在OP 50- NG M 培养基中生长良好, 在JM 109-L B 培养基中生长较差。在-80 ℃最佳温度条件下,秀丽隐杆线虫在含 50 m L/L 甘油的LB 培养基中的存活率达7 8%;在含50 m L/L 甘油的PBS 中的存活率达52%;而在含100 mL/L 甘油的水溶液中的存活率仅为8%。用液氮保存,成虫和幼虫均可存活;而在-80 ℃和-20 ℃条件 下,仅有幼虫存活。结果表明,秀丽隐杆线虫在OP50-N G M 培养基中培养发育生长较好;在-80 ℃条件下, 含50 m L/L 甘油的LB 培养基适于保存秀丽隐杆线虫。 关键词:秀丽隐杆线虫;培养;保存 Study on the culture and preservation of Caenorhabditis eleg ans LI Yo u-quan,GUA N Gui-quan,P EN G Yu-ǜ, H E H ai-ni ng, L U O Jian-xun, YIN H ong (Key Laboratory of Veterinary Parasitology of Gansu Province/State Key Laboratory of Veterinary Etiological Biology/ Lanzhou Veterinary Research Institute, Chinese Acade y of Agricultural Sciences, Lanz hou 730046, China) 　　Abstract:Grow t h eff ect of Caenorhabditis elegans on four diff erent medium i nc uding OP50-N G M, JM 109-N GM , OP50-LB and JM 109-L B w ere eva uat ed and compared w it h each o ther .T he pre serving ef fect s of C.elegans i n t hree preserving so utions,w ith dif ferent proporti ona g yce ro w ere eva uated under the conditio n o f iquid nit rog en -80 ℃and -20 ℃, respect ive y .Resu t s show ed that C.elegans g rew bet- ter on t he NG M m edium covered w it h E.coli OP50,t han o ther t hree m edium s.F or preserving so ut ion, C. elegans had a surviva rate of 78 %in LB medium containi ng 50 mL/L g yce ro at -80 ℃and it had a sur- viv a rate of 52 %in PBS at t he same conditio n as LB medium, w hi e in w ate r w ith 100