slo-1-channels of parasitic nematodes reconstitute locomotor behaviour and emodepside sensitivity in caenorhabditis elegans slo-1 loss of function mutantsslo-1-channels寄生线虫的运动行为和重组emodepside敏感性秀丽隐杆线虫slo-1失去功能的突变体.pdf

SLO-1-Channels of Parasitic Nematodes Reconstitute Locomotor Behaviour and Emodepside Sensitivity in Caenorhabditis elegans slo-1 Loss of Function Mutants 1. ¨ 2. 1 2 ¨ ¨ 2 Claudia Welz , Nina Kruger , Monika Schniederjans , Sandra M. Miltsch , Jurgen Krucken , Marcus 3 3 4 2 Guest , Lindy Holden-Dye , Achim Harder , Georg von Samson-Himmelstjerna * ¨ 1 Institute for Parasitology, University of Veterinary Medicine Hannover, Hannover, Germany, 2 Institute for Parasitology and Tropical Veterinary Medicine, Freie Universitat Berlin, Berlin, Germany, 3 School of Biological Sciences, University of Southampton, Southampton, United Kingdom, 4 Bayer HealthCare AG, Animal Health, RD- Parasiticides, Monheim, Germany Abstract The calcium-gated potassium channel SLO-1 in Caenorhabditis elegans was recently identified as key component for action of emodepside, a new anthelmintic drug with broad spectrum activity. In this study we identified orthologues of slo-1 in Ancylostoma caninum, Cooperia oncophora, and Haemonchus contortus, all important parasitic nematodes in veterinary medicine. Furthermore, functional analyses of these slo-1 orthologues were performed using heterologous expression in C. elegans. We expressed A. caninum and C. oncophora slo-1 in the emodepside-resistant genetic background of the slo-1 loss- of-function mutant NM1968 slo-1(js379). Transformants expressing A. caninum slo-1 from C. elegans slo-1 promot