crystal structure of ehec intimin insights into the complementarity between epec and ehec晶体结构的肠出血性大肠杆菌intimin见解之间的互补性epec和肠出血性大肠杆菌.pdf

Crystal Structure of EHEC Intimin: Insights into the Complementarity between EPEC and EHEC Yong Yi1,3,5., Ying Ma1,2., Feng Gao6., Xuhu Mao1., Hao Peng2,5, Youjun Feng2,4,5, Zheng Fan2,5, Guihua 2,4,5 1 2,5 1 1 2,5,7 Wang , Gang Guo , Jinghua Yan , Hao Zeng , Quanming Zou *, George F. Gao * 1 Department of Clinical Microbiology and Clinical Immunology, College of Medical Laboratory, Third Military Medical University, Chongqing, China, 2 CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China, 3 The 306th Hospital of PLA, Beijing, China, 4 Graduate University, Chinese Academy of Sciences, Beijing, China, 5 China-Japan Joint Laboratory of Molecular Immunology and Molecular Microbiology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China, 6 National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China, 7 Beijing Institutes of Life Science, Chinese Academy of Sciences, Beijing, China Abstract Enterohaemorrhagic E. coli (EHEC) O157:H7 is a primary food-borne bacterial pathogen capable of causing life-threatening human infections which poses a serious challenge to public health worldwide. Intimin, the bacterial outer-membrane protein, plays a key role in the initiating process of EHEC infection. This activity is dependent upon translocation of the intimin receptor (Tir), the intimin binding partner of the bacteria-encoded host cell surface protein. Intimin has attracted considerable attention due to its potential function as an antibacterial drug target. Here, we report the crystal structure of