spectrometric and voltammetric analysis of urease – nickel nanoelectrode as an electrochemical sensor光谱和脲酶伏安分析u2014u2014镍nanoelectrode作为电化学传感器.pdf

Sensors 2007, 7, 1238-1255 sensors ISSN 1424-8220 © 2007 by MDPI /sensors Full Paper Spectrometric and Voltammetric Analysis of Urease – Nickel Nanoelectrode as an Electrochemical Sensor Jaromir Hubalek 1, Jan Hradecky 2, Vojtech Adam 2,3, Olga Krystofova 2, Dalibor Huska 2, 4 5 6 1 1 Michal Masarik , Libuse Trnkova , Ales Horna , Katerina Klosova , Martin Adamek , Josef Zehnalek 2 and Rene Kizek 1,* 1 Department of Microelectronics, Faculty of Electrical Engineering and Communication, Brno University of Technology, Udolni 53, CZ-602 00 Brno, Czech Republic 2 Department of Chemistry and Biochemistry, and 3 Department of Animal Nutrition and Forage Production, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Zemedelska 1, CZ- 613 00 Brno, Czech Republic 4 Department of Pathological Physiology, Faculty of Medicine, Masaryk University, Komenskeho namesti 2, CZ-662 43 Brno, Czech Republic 5 Department of Chemistry, Faculty of Science, Masaryk University, Kotlarska 2, CZ-611 37 Brno, Czech Republic 6 Department of Food Engineering, Faculty of Technology, Tomas Bata University, T.G. Masaryka 275, CZ-762 72 Zlin, Czech Republic * Author to whom correspondence should be addressed; E-mail: kizek@sci.muni.cz Received: 3 July 2007 / Accepted: 13 July 2007 / Published: 16 July 2007 Abstract: Urease is the enzyme catalyzing the hydrolysis of urea into carbon dioxide and ammonia. This enzyme is substrate-specific, which mea