the anti-cyclic citrullinated peptide response in tuberculosis patients is not citrulline-dependent and sensitive to treatment的anti-cyclic citrullinated肽反应对治疗肺结核病人不是citrulline-dependent和敏感.pdf

Elkayam et al. Arthritis Research Therapy 2010, 12:R12 /content/12/1/R12 R E S E A R C H A R T I C L E Open Access Research article The anti-cyclic citrullinated peptide response in tuberculosis patients is not citrulline-dependent and sensitive to treatment †1 †2 3 4 5 5 Ori Elkayam* , Refael Segal , Daniele Bendayan , Robert van Uitert , Carla Onnekink and Ger JM Pruijn Abstract Introduction: Patients with tuberculosis (TB) frequently produce anti-citrullinated protein antibodies (ACPA). The objective of this study is to characterize the citrulline-dependence of the ACPA reactivity in sera of patients with mycobacterium infections. Methods: Serum samples of 134 patients with untreated mycobacterium infections (122 TB, 12 nontuberculous mycobacterium) were tested for antibodies against both the citrullinated (Cit) and the non-citrullinated (Arg) form of 2 cyclic synthetic peptides. In 33 patients, a follow-up sample was tested six months after starting anti-mycobacterial drugs. Results: A substantial proportion of patients with mycobacterial infections demonstrated antibodies against 0401Cit, 0401Arg, 0722Cit and 0722Arg. Fourteen patients demonstrated anti-0401Cit, 83 anti-0401Arg, 22 anti-0722Cit and 61 anti-0722Arg, while none of these antibodies were detected in the 20 healthy controls. All the patients but one, who were anti-0401Cit and anti-0722Cit positive, demonstrated reactivity against the respective Arg peptide. In the subset of 33 patients with a follow-up test six months after starting treatment, the mean levels of antibodies to 0401Cit, 0401Arg, 0722Cit and 0722Arg significantly decreased after treatment. All the patients who were anti-0401Cit and anti- 0722Cit positive turn