biochip-based detection of kras mutation in non-small cell lung cancerbiochip-based kras突变检测在非小细胞肺癌.pdf

Int. J. Mol. Sci. 2011, 12, 8530-8538; doi:10.3390/ijm OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article Biochip-Based Detection of KRAS Mutation in Non-Small Cell Lung Cancer Gernot Kriegshäuser 1, Gerhild Fabjani 2,3, Barbara Ziegler 2, Sabine Zöchbauer-Müller 4, Adelheid End 5 and Robert Zeillinger 2,3,* 1 ViennaLab Diagnostics GmbH, 1120 Vienna, Austria; E-Mail: kriegshauser@viennalab.co.at 2 Molecular Oncology Group, Department of Obstetrics and Gynecology, Medical University Vienna, 1090 Vienna, Austria; E-Mails: gerhild.fabjani@gmx.at (G.F.); barbara.ziegler@meduniwien.ac.at (B.Z.) 3 Ludwig Boltzmann Gesellschaft, Cluster Translational Oncology, 1090 Vienna, Austria 4 Division of Oncology, Department of Internal Medicine I, Medical University of Vienna, 1090 Vienna, Austria; E-Mail: sabine.zoechbauer-mueller@meduniwien.ac.at 5 Department of Cardiothoracic Surgery, Medical University of Vienna, 1090 Vienna, Austria; E-Mail: adelheid.end@meduniwien.ac.at * Author to whom correspondence should be addressed; E-Mail: robert.zeillinger@meduniwien.ac.at; Tel.: +431404007831; Fax: +431404002820. Received: 18 September 2011; in revised form: 17 November 2011 / Accepted: 17 November 2011 / Published: 29 November 2011 Abstract: This study is aimed at evaluating the potential of a biochip assay to sensitively detect KRAS mutation in DNA from non-small cell lung cancer (NSCLC) tissue samples. The assay covers 10 mu