targeting a newly established spontaneous feline fibrosarcoma cell line by gene transfer针对新成立的自发的猫科动物纤维肉瘤细胞系基因转移.pdf

Targeting a Newly Established Spontaneous Feline Fibrosarcoma Cell Line by Gene Transfer Rounak Nande1,2, Altomare Di Benedetto1,2,3., Pierpaolo Aimola1,2,3., Flavia De Carlo1,2, 1,2 1,2 1,2 4 5 Miranda Carper , Charlene D. Claudio , Jim Denvir , Jagan Valluri , Gary C. Duncan , Pier Paolo Claudio1,2,6* 1 Department of Biochemistry and Microbiology, Joan C. Edwards School of Medicine, Marshall University, Huntington, West Virginia, United States of America, 2 McKown Translational Research Institute, Edwards Comprehensive Cancer Center, Huntington, West Virginia, United States of America, 3 Department of Basic and Applied Biology, Faculty of Sciences, University of L’Aquila, L’Aquila, Italy, 4 Department of Biological Sciences, Marshall University, Huntington, West Virginia, United States of America, 5 Martin Veterinary Clinic, Ashland, Kentucky, United States of America, 6 Department of Surgery, Joan C. Edwards School of Medicine, Marshall University, Huntington, West Virginia, United States of America Abstract Fibrosarcoma is a deadly disease in cats and is significantly more often located at classical vaccine injections sites. More rare forms of spontaneous non-vaccination site (NSV) fibrosarcomas have been described and have been found associated to genetic alterations. Purpose of this study was to compare the efficacy of adenoviral gene transfer in NVS fibrosarcoma. We isolated and characterized a NVS fibrosarcoma cell line (Cocca-6A) from a spontaneous fibrosarcoma that occurred in a domestic calico cat. The feline cells were karyotyped and their chromosome number was counted using a Giemsa staining. Adenoviral gene transfer was verified by western blot analysis. Flow cytometry assay and Annexin-V were used to study cell-