uncovering genes with divergent mrna-protein dynamics in streptomyces coelicolor揭示基因与发散mrna-protein动力学链霉菌属coelicolor.pdf

Uncovering Genes with Divergent mRNA-Protein Dynamics in Streptomyces coelicolor 1 2 2 2 3,4,5 Karthik P. Jayapal , Robin J. Philp , Yee-Jiun Kok , Miranda G. S. Yap , David H. Sherman , Timothy J. 6 1 Griffin , Wei-Shou Hu * 1 Department of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis, Minnesota, United States of America, 2 Bioprocessing Technology Institute, Agency for Science Technology and Research, Singapore, Singapore, 3 Life Sciences Institute, Department of Medicinal Chemistry, University of Michigan, Ann Arbor, Michigan, United States of America, 4 Life Sciences Institute, Department of Chemistry, University of Michigan, Ann Arbor, Michigan, United States of America, 5 Life Sciences Institute, Department of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan, United States of America, 6 Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, Minnesota, United States of America Abstract Many biological processes are intrinsically dynamic, incurring profound changes at both molecular and physiological levels. Systems analyses of such processes incorporating large-scale transcriptome or proteome profiling can be quite revealing. Although consistency between mRNA and proteins is often implicitly assumed in many studies, examples of divergent trends are frequently observed. Here, we present a comparative transcriptome and proteome analysis of growth and stationary phase adaptation in Streptomyces coelicolor, taking the time-dynamics of process into consideration. These processes are of immense interest in microbiology as they pertain to the physio