响应曲面法优化蓝藻多糖提取工艺研究学士学位论文.doc

安徽大学 本科毕业论文 题　　目：学生姓名：　 学号： 院（系）： 　生命科学学院　 专业： 生物 入学时间：　 　　20 　 　年　 09 　月 导师姓名：　 孔小卫　　 职称/学位： 副教授 导师所在单位：　　 　安徽大学生命科学学院  作者：周维清 安徽大学生命科学学院2010级生物工程专业，230601 指导教师：戴玲 孔小卫 安徽大学生命科学学院，230601 摘要 关键词：Optimization of the extraction technology of algae’Polysaccharides by Response Surface Methodology Author: ZhouWeiqing School:Anhui University Hefei, 230601 Department: School of Life Sciences Major:2010 Biological Engineering, Hefei, 230601 Advisor: Kong Xiaowei and DaiLing School of Life Sciences,in Anhui University Hefei, 230601 Abstract Objective: the?extraction technology of? algae’polysaccharide by using response surface analysis? Method: On the basis of?extraction temperature,?extraction time,?ratio of material to liquid,?freeze thawing cycles,?lysozyme?and EDTA experiment of 6 single factor, ?choose extraction temperature,?extraction time,?solid to liquid ratio of 3 factors and 3 levels to conduct Study of Box?-Behnken center?, and analyze the test data?by use Design?Expert?8.0.Establish a mathematical model of effect on extraction?rate?of?polysaccharide?about the?above 3?main factors, finally,the?extraction?conditions were optimized?by response surface analysis,?and determined the optimal condition?of blue-green algae?polysaccharide extraction?. Results: the?influence factors on the?sequence?of Microcystis?aeruginosa?polysaccharide?extraction ?are ? as? follows: extracting?temperaturesolid-liquid ratio?extraction time. Conclusion: the?optimum extraction conditions:?extraction temperature 53 ℃,solid-liquid ratio 1:51,?extraction time 4.3h,?under this condition,?the crude polysaccharide?expected?rate of 1.18%,?the actual?rate of 1.06%. Keywords:?algae;?water?soluble polysaccharide;?response surface analysis;process optimization 目 录 1引言 1 1.1铜绿微囊藻的研究背景 1 1.2铜绿微囊藻多糖研究背景 1 1.3响应曲面法的研究进展 2 1.4论文研究的意义 2 2.材料、试剂和仪器 2 2.1材料 2 2.2试剂 3 2.3 仪器设备 3 3 方法 3 3.1蓝藻多糖提取工艺路线 3 3.2蓝藻多糖单因素提取实验 3 3.3蓝藻多糖提取的中心组合实验 3 3.4 数据处理 4 4 结果与分析 4 4.1 单因素提取对水溶性多糖得率的