the early postnatal nonhuman primate neocortex contains self-renewing multipotent neural progenitor cells产后早期非人类灵长类动物大脑皮层包含自我更新的多功能神经祖细胞.pdf

The Early Postnatal Nonhuman Primate Neocortex Contains Self-Renewing Multipotent Neural Progenitor Cells 1 1,2.* 1.* Jihane Homman-Ludiye , Tobias D. Merson , James A. Bourne 1 Australian Regenerative Medicine Institute, Monash University, Clayton, Victoria, Australia, 2 Florey Neuroscience Institutes and Centre for Neuroscience, University of Melbourne, Melbourne, Victoria, Australia Abstract The postnatal neocortex has traditionally been considered a non-neurogenic region, under non-pathological conditions. A few studies suggest, however, that a small subpopulation of neural cells born during postnatal life can differentiate into neurons that take up residence within the neocortex, implying that postnatal neurogenesis could occur in this region, albeit at a low level. Evidence to support this hypothesis remains controversial while the source of putative neural progenitors responsible for generating new neurons in the postnatal neocortex is unknown. Here we report the identification of self- renewing multipotent neural progenitor cells (NPCs) derived from the postnatal day 14 (PD14) marmoset monkey primary visual cortex (V1, striate cortex). While neuronal maturation within V1 is well advanced by PD14, we observed cells throughout this region that co-expressed Sox2 and Ki67, defining a population of resident proliferating progenitor cells. When cultured at low density in the presence of epidermal growth factor (EGF) and/or fibroblast growth factor 2 (FGF-2), dissociated V1 tissue gave rise to multipotent neurospheres that exhibited the ability to differentiate into neurons, oligodendrocytes and astrocytes. While the capacity to generate neurones and oligodendrocytes was not observed beyond the third passag