unconventional cadherin localization in honey bee gonads revealed through domain-specific apis mellifera e- and n-cadherin antibodies indicates alternative functions蜜蜂性腺的非传统的钙粘着蛋白定位显示通过特定于域的蜜蜂e -和n-cadherin抗体表明替代功能.pdf

Insects 2012, 3, 1200-1219; doi:10.3390/insects3041200 OPEN ACCESS insects ISSN 2075-4450 /journal/insects/ Article Unconventional Cadherin Localization in Honey Bee Gonads Revealed Through Domain-Specific Apis mellifera E- and N-Cadherin Antibodies Indicates Alternative Functions Mônica M. Florecki and Klaus Hartfelder * Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Avenida Bandeirantes 3900, Ribeirão Preto 14049-900, SP, Brazil; E-Mail: mmflorec@ * Author to whom correspondence should be addressed; E-Mail: klaus@fmrp.usp.br; Tel.: +55-16-3602-3063; Fax: +55-16-3630-1786. Received: 13 August 2012; in revised form: 2 November 2012 / Accepted: 6 November 2012 / Published: 22 November 2012 Abstract: As key factors in intercellular adhesion processes, cadherins play important roles in a plethora of developmental processes, including gametogenesis. In a previous study on cadherin localization in the gonads of honey bees, performed with heterologous pan-cadherin antibodies, we detected these proteins as (i) associated with cell membranes, (ii) as homogeneously distributed throughout the cytoplasm, and (iii) as nuclear foci in both somatic and germline cells, raising the possibility of alternative functions. To further investigate such unusual intracellular cadherin localization we produced specific antibodies against the N- and C-terminal domains of honey bee N-