β-sitosterol supercritical carbon dioxide extraction from sea buckthorn (hippophae rhamnoides l.) seedsβ-sitosterol超临界二氧化碳萃取沙棘(沙棘l .).pdf

Int. J. Mol. Sci. 2010, 11, 1842-1850; doi:10.3390/ijm OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article -Sitosterol: Supercritical Carbon Dioxide Extraction from Sea Buckthorn (Hippophae rhamnoides L.) Seeds Marie Sajfrtová 1, Ivana Ličková 1,2, Martina Wimmerová 3, Helena Sovová 1 and Zdeněk Wimmer 3,* 1 Institute of Chemical Process Fundamentals of the AS CR, v.v.i., Rozvojová 135/2, 165 00 Prague 6, Czech Republic; E-Mails: sajfrtova@icpf.cas.cz (M.S.); Lickova.Iva@seznam.cz (I.L.); sovova@icpf.cas.cz (H.S.) 2 Institute of Chemical Technology Prague, Technická 5, 166 28 Prague 6, Czech Republic 3 Institute of Experimental Botany of the AS CR, v.v.i., Isotope Laboratory, Vídeňská 1083, 142 20 Prague 4, Czech Republic; E-Mail: wimmerova@biomed.cas.cz (M.W.) * Author to whom correspondence should be addressed; E-Mail: wimmer@biomed.cas.cz; Tel.: +420-241-062-457; Fax: +420-241-062-150. Received: 3 February 2010 / Accepted: 20 April 2010 / Published: 22 April 2010 Abstract: Supercritical fluid extraction represents an efficient and environmentally friendly technique for isolation of phytosterols from different plant sources. Sea buckthorn (Hippophae rhamnoides L.) seeds were extracted with supercritical carbon dioxide at pressures ranging from 15–60 MPa and temperatures of 40-80 °C. Oil and -sitosterol yields were measured in the extractio