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BMC Physiology BioMed Central Research article Open Access Takifugu obscurus is a euryhaline fugu species very close to Takifugu rubripes and suitable for studying osmoregulation 1 2 1 3 Akira Kato , Hiroyuki Doi , Tsutomu Nakada , Harumi Sakai and Shigehisa Hirose*1 Address: 1Department of Biological Sciences, Tokyo Institute of Technology, Yokohama, Japan, 2Shimonoseki Marine Science Museum Kaikyokan, Shimonoseki Academy of Marine Science, Shimonoseki, Japan and 3Department of Applied Aquabiology, National Fisheries University, Shimonoseki, Japan Email: Akira Kato - akirkato@bio.titech.ac.jp; Hiroyuki Doi - doi@; Tsutomu Nakada - tnakada@bio.titech.ac.jp; Harumi Sakai - sakaih@fish-u.ac.jp; Shigehisa Hirose* - shirose@bio.titech.ac.jp * Corresponding author Published: 20 December 2005 Received: 09 July 2005 Accepted: 20 December 2005 BMC Physiology 2005, 5:18 doi:10.1186/1472-6793-5-18 This article is available from: /1472-6793/5/18 © 2005 Kato et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: The genome sequence of the pufferfish Takifugu rubripes is an enormously useful tool in the molecular physiology of fish. Euryhaline fish that can survive both in freshwater (FW) and