细菌发光传感器在快速检测污染物急性毒性中的应用-环境科学.PDF

1997 7 EN V IRO N M ENT A L SCIEN CE J uly , 1997 * 黄　正　汪亚洲　王家玲 ( , 430030) , , . . 3 3 ( 50% EC 50 ) , . pH7. 0, 20 , 3. 0% N a Cl , 250 - 30010- 7 mW , 60- 80m in; EC 50 ( mg/ L) Hg2 + ( 0. 15) Cu 2+ ( 14) Zn 2+ ( 130) , ( 35) ( 210) ( 1200) , L D 50 , ; . , , , , , . [ 1, 2] , 1 , [ 3] 1. 1 , DJ - ( , ( M icro to x) [ 4] ( 15m in) 23 ) , CS50 1 ( ( ) , ( Melsungen ) . 1995 GA ) , 78HW -( ) , . ( G B/ T 1544 1-1995) [ 5] . 1. 2 , ( P ho to bacterium pho spho - , reum ) . . A SW ( W / V ) ( 0. 5% ) , ( 0. 5 % ) , ( 3% ) , , ( 0 . 5% ) , ( 0. 1% ) , ( 0. 3% ) , , BO D ( 1. 5% ) , pH 7. 0 [ 6] . 1. 3 HgCl , Cu SO4 5H 2O , ZnSO4 7H2 O , , C6H6 O , C2H4O , C4H 8O2 . . 1. 4 , , A SW , 20 , , , 200 / 12 , 108 r min h . * 1997-03-10 4 15 / ml , 5m l . 25mm , 0. 65m A SW , , , ( ) , 2 , 1. 2. 3. 4. 5. 6. 7. ( 1) . 5% ) 60- 80min .