一株裂解性低温假单胞菌噬菌体的分离及特性研究.pdf

18 卷 3 期 生命科学研究 灾燥造援员8 晕燥援3 圆园14 年6 月 蕴蚤枣藻 杂糟蚤藻灶糟藻 砸藻泽藻葬则糟澡 Jun. 圆园14 一株裂解性低温假单胞菌噬菌体的分离及特性研究 策 赵 龙 魏云林 林连兵 张 琦 季秀玲* （昆明理工大学生命科学与技术学院，中国云南 昆明650500 ） 摘 要 从明永冰川低温土壤中分离尧纯化获得一株低温假单胞菌噬菌体PFV1 并对其生物学特性(噬菌体基 因组限制性酶切片段长度多态性尧衣壳蛋白组分分析及生理特征)进行了初步研究遥基于 16S rRNA 基因测序分 析结果 将宿主菌初步鉴定为荧光假单胞菌菌株遥 噬菌体PFV1 为球形 直径约50 nm遥 PFV1 在4 时具有 染活 性 4~25 范围内均可形成噬菌遥 对氯仿具有一定的敏感性 具有热不稳定性 基因组为双链DNA 大小约38 kb遥 关键词 荧光假单胞菌曰低温噬茵体曰分离曰特性 中图分类号 Q93 文献标识码 A 文章编号 1007-7847 渊2014 冤03-0222-05 Isolation and Characterization of a Lytic Cold-active Bacteriophage of Pseudomonas f luorescens SUN Ce ，ZHAO Long ，WEI Yun-lin ，LIN Lian-bing ，ZHANG Qi ，JI Xiu-ling* （Faculty of L if e Sc ience and Technology, K unming Unive rs ity of Science and Technology, K unming 650500, Yunnan, China） Abstract ：A lytic cold-active bacteriophage, designated as PFV 1, was isolated from soil sample of Mingyong glacier.Restriction fragment length polymorphism analysis of genomic DNA, constituent proteins and physio原 logical analysis of PFV 1 were further carried out to characterize the phage. Based on the 16S rRNA gene se原 quence, the host strain was identified as a member of Pseudomonas f luorescens . The morphology of the iso原 lated phage was observed by electron microscope. The bacteriophage is spherical, about 50 nm in diameter. Physiological analysis results showed that PFV 1 had infection activity at 4 , and plaques were formed be原 tween 4 and 25 . It was sensitive to chloroform, and showed thermolability. Furthermore, the genome of PFV 1 consists of double-stranded DNA and is approximately 38 kb. Key words ：Pseudomonas fl uorescens ；cold-ac