线虫发育及基因功能研究韩珉.pptx

线虫发育及基因功能研究韩珉;Pathway in early 1992;R7;Strategy: moving from RTK down: discovering SOS;Son of Sevenless: hyperactive mutation;Drosophila: F1 screen vs F2 screen;Mike Simon : landmark modify screen;SOS encodes an exchange factor for GTPase;第9页/共45页;Moving from receptor down: biochemistry;Discovery of GRB2;Discovery of GRB2: complementary works by biochemists and geneticists;The sem-5 story - early 1992;The rest of the story is there in a rush and clash- mid 1993;Many Small GTPases have a conserved C-terminus;Ras is lipid modified for its membrane binding;;Half way done;Summary of the original screens;The holy grail: what is the effector of Ras?;The Ras effector;The Ras effector domain is defined by ;GAP = Ras effector;GAP = Ras effector;Key argument;The field was convinced;The field was convinced;The field was convinced;The field was convinced;Functions;Genetics;What did genetics say;The end of 1992: GAP was no longer considered a Ras effector;The Story of Raf;The Story of Raf;The Story of Raf: summer 1993;Main data in these six papers;Late 1992 and early 1993;Compare Raf with GAP;What is the biological function of Ras-Raf binding?;Making a constitutive Raf kinase;Genetics to identify factors downstream of Ras;Genetics to identify factors downstream of Ras;Genetics to identify factors downstream of Ras;谢谢观看！