《先张法预应力溷凝土简支空心板设计》.doc

先张法预应力混凝土简支空心板设计 本桥设计荷载等级确定为汽车荷载（公路—I级），人群荷载为3.5KN/m2 标准跨径：Lk=20m 计算跨径：L=19.50 m 桥面净宽：净—9.0+2×0.75m 主梁全长： 19.96m。 ... p内容简介/p p本桥设计荷载等级确定为汽车荷载（公路mdash;I级），人群荷载为3.5KN/m2 标准跨径：Lk=20m 计算跨径：L=19.50 m 桥面净宽：净mdash;9.0+2times;0.75m 主梁全长： 19.96m。 /p pnbsp; p class=Zhi529/p /p p class=MsoNormal style=margin: 0cm 0cm 0ptspan style=font-family: 宋体; mso-ascii-font-family: Times New Roman; mso-hansi-font-family: Times New Roman正文（/spanspan lang=EN-US29/spanspan style=font-family: 宋体; mso-ascii-font-family: Times New Roman; mso-hansi-font-family: Times New Roman页）、/spanspan lang=EN-USCAD/spanspan style=font-family: 宋体; mso-ascii-font-family: Times New Roman; mso-hansi-font-family: Times New Roman图纸（/spanspan lang=EN-US2/spanspan style=font-family: 宋体; mso-ascii-font-family: Times New Roman; mso-hansi-font-family: Times New Roman张）/span font color=#9a9a9a/font /p p class=MsoNormal style=margin: 0cm 0cm 0ptspan style=font-family: 宋体; mso-ascii-font-family: Times New Roman; mso-hansi-font-family: Times New Roman目录：/span /pP/P p摘 要: 根据细菌的16S rDNA-23S rDNA的高度保守区设计引物，扩增了嗜肺巴斯德杆菌的16S-23S rDNA间区，经测序获得521bp和683bp两条产物。用BLAST和DNAster软件对16S-23S rDNA间区序列进行比较分析，设计出对嗜肺巴斯德杆菌特异的PCR引物，进行了特异性、灵敏性检测分析。为建立检测嗜肺巴斯德杆菌的PCR方法奠定了良好的基础。br / 关健词: 嗜肺巴斯德杆菌；16S-23S rDNA间区；特异性；灵敏性br / br / Establishment and application of Pasteurella pneumotropica coli 16S rDNA-PCR detection methods br / Abstract: In this study, PCR primer Designed from 16S rDNA-23S rDNA conserved Intergenic Spacer Region and amplified this region, and obtained PCR products of 521bp and 683bp. These products sequenced. Using BLAST and DNAstar software to conduct comparison analysis to 16S-23S rDNA PCR products specific to Pasteurela pneumotropica, then PCR primers was conducted testing analysis of specificity and sensitivity. The purpose to make a good foundation to establish PCR-based method ofnbsp; detection to Pasteurela pneumotropica.br / Key words: Pasteurela pneumotropica; 16S-23S rDNA Sequence; Specificity; Sensitivity font color=#9a9a