铬污染土壤修复过程中土壤细菌群落多样性的rflp分析_翟文.pdf

37 4 ( ) Vol. 37 No. 4 2009 4 Journal of Northwest A F University( Nat. S i. Ed. ) Apr . 2009 * RFLP a a, b a 翟 文, 王保莉 , 曲 东 , 李 杨 ( a , b , , 712100) [ ] = = , DNA, 16S rDNA , PCR-RFLP , 10 d( , S1) Cr( Ö) 10 d( S2) Cr ( Ö ) Fe( OH) 10 d( S3) 20 d( S4) 4 = 16S rDNA , 3 ; Rsa Ñ16S rDNA PCR-RFLP , 123, 120, 97 69 , 54. 92% , 55. 43% , 65. 33% 76. 60% ; Shannon-W iener Gini ( dMa ) (J gi) S1 S2 S3 S4, 4 11. 51% , 1. 84% , 23. 64% 1. 55% ; , S1 Cr ( Ö) S2 , 2 Fe S3 S4 = 10 d , Cr ( Ö) 98% , Cr ( Ö) ( S2) ( S1) , 2 ( S3 S4) , S1 S2; ( S3 S4) , , [ ] ; ; ; ; 16S rDNA ; RFLP [ ] X53; X172 [ ] A [ ] 167 1-9387( 2009) 04-0128-07 RFLP analysis bacterial communit ies of remedied chromium- contaminated soil by different methods a a, b a ZH AI Wen , WANG Bao-li , QU Dong , LI Yang ( a Coll eg e of L if e S cie nces , b S ta te K ey L aborat or y of S o i l Er os i on and D r y l and Far ming on L oe ss P lat eau c Col le ge of R eso urce and Env i ronment , N or th w est A F Univ ers i ty , Yang li ng , S haanx i 712100, Chi na) Abstract: =Obje tive T he hanges of soil ba terial ommunity w ere studied during the pro ess of soil remediation. =M ethod T he simulation Cr( Ö) ontaminated soil w as used as test mater ial, w hi h had been tr eated by w aterlogging for