石榴皮多酚超高压提取物的纯化_分离及功能性研究_严陇兵.doc

------------------------------------------------------------------------------------------------ —————————————————————————————————————— 石榴皮多酚超高压提取物的纯化_分离及功能性研究_严陇兵 ScienceandTechnologyofFoodIndustry 研究与探讨 石榴皮多酚超高压提取物的纯化、分离 及功能性研究 严陇兵，刘邻渭*，林静雅，徐云凤 （西北农林科技大学食品科学与工程学院，陕西杨凌712100） 摘 要：为探明超高压提取石榴皮多酚的组分和功能特性，研究了石榴皮多酚超高压提取物的大孔树脂纯化和有机 溶剂分级萃取，并对提取物、纯化物和分级产物的体外抗氧化活性和抑菌性能进行了测定。结果表明：大孔树脂纯化物的总酚含量高达77.40%，且抗氧化活性和抑菌性能均有显著提高；HPLC测定结果显示，大孔树脂纯化未改变多酚种类；有机溶剂分级萃取法能有效地将纯???物进一步分离，其中正丁醇相的萃取得率达到55.28%；不同萃取相产物的总酚含量大小顺序为：正丁醇相＞乙酸乙酯相＞水相，它们的抗氧化活性强弱顺序为：乙酸乙酯相＞正丁醇相＞水相；正丁醇相产物的抑菌活性最高，显著高于水相和乙酸乙酯相。关键词：石榴皮多酚，超高压，抗氧化，抑菌 Studyonthepurification，separationandfunctionsof polyphenolextractionsbyultrahighpressurefrompomegranatepeel YANLong-bing，LIULin-wei*，LINJing-ya，XUYun-feng （CollegeofFoodScienceandEngineering，NorthwestAgricultureandForestryUniversity，Yangling712100，China）Abstract：Toascertaintheantioxidativeandantimicrobialactivitiesofpolyphenolextractedbytheultrahighpressureprocessing（UHPP）frompomegranatepeel，basedonpurificationbymacroporousabsorbentresinandseparationbyorganicsolvents，extractsandsampleswithdifferentpurityquotientsandpolaritieswereobtainedandstudied.Theresultshowedthattotalpolyphenolcontentofpurificationproductwas77.40%，andtheantioxidativeandantimicrobialactivitieswereimprovedsignificantly，HPLCshowedthatthetypesofpolyphenolcompoundswerenotchangedafterpurification，thepurificationsamplehadbeensuccessfullyseparatedbyorganicsolvent，theyieldofn-butanolphaseattainedto55.28%，thesequenceoftotalpolyphenolcontentsindifferentextractionphasesfromhighesttolowestwasn-butanolphase＞ethylacetatephase＞waterphase，thesequenceofantioxidativeactivitywasethylacetatephase＞n-butanolphase＞waterphase，then-butanolphasehadthehighestantimicrobialactivitywhichwassignificantlyhigherthanwaterandethylacetatephase. Keywords：polyphenolsfrompomegranatepeel；ultrahighpressure；antioxidativeactivity；antimicrobialactivity中图分类号：TS209文献标识码：A文章编号：1002-0306（2012）22-0168-05石榴（Pu