ubiquitous [na+]i[k+]i-sensitive transcriptome in mammalian cells evidence for ca2+i-independent excitation-transcription coupling无处不在的[na +]我[k +]i-sensitive在哺乳动物细胞中转录组证据ca2 + i-independent excitation-transcription耦合.pdf

+ + Ubiquitous [Na ] /[K ] -Sensitive Transcriptome in i i Mammalian Cells: Evidence for Ca2+i-Independent Excitation-Transcription Coupling 1,2 3 1 2,3 1,4 Svetlana V. Koltsova , Yulia Trushina , Mounsif Haloui , Olga A. Akimova , Johanne Tremblay , Pavel Hamet1,4, Sergei N. Orlov1,4* ´ ´ ˆ 1 Centre de recherche, Centre hospitalier de l’Universite de Montreal (CRCHUM) – Technopole Angus, Montreal, PQ, Canada, 2 Institute of General Pathology and Pathophysiology, Russian Academy of Medical Sciences, Moscow, Russia, 3 Faculty of Biology, M.V. Lomonosov Moscow State University, Moscow, Russia, 4 Department of ´ ´ Medicine, Universite de Montreal, Montreal, PQ, Canada Abstract Stimulus-dependent elevation of intracellular Ca2+ ([Ca2+] ) affects the expression of numerous genes – a phenomenon i known as excitation-transcription coupling. Recently, we found that increases in [Na+] trigger c-Fos expression via a novel i 2+ + + Ca -independent pathway. In