t4-related bacteriophage limestone isolates for the control of soft rot on potato caused by ‘dickeya solani’t4-related噬菌体石灰岩隔离对马铃薯软腐病的控制造成的dickeya以上的.pdf

T4-Related Bacteriophage LIMEstone Isolates for the Control of Soft Rot on Potato Caused by ‘Dickeya solani’ 1,2,3 2 1 1 Evelien M. Adriaenssens , Johan Van Vaerenbergh , Dieter Vandenheuvel , Vincent Dunon , Pieter- 1 3 4,5 6 2 Jan Ceyssens , Maurice De Proft , Andrew M. Kropinski , Jean-Paul Noben , Martine Maes , Rob Lavigne1* 1 Division of Gene Technology, Katholieke Universiteit Leuven, Heverlee, Belgium, 2 Unit Plant – Crop Protection, Institute for Agricultural and Fisheries Research, Merelbeke, Belgium, 3 Division of Crop Biotechnics, Katholieke Universiteit Leuven, Heverlee, Belgium, 4 Laboratory of Foodborne Zoonoses, Public Health Agency of Canada, Guelph, Ontario, Canada, 5 Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada, 6 Biomedical Research Institute and Transnational University Limburg, School of Life Sciences, Hasselt University, Diepenbeek, Belgium Abstract The bacterium ‘Dickeya solani’, an aggressive biovar 3 variant of Dickeya dianthicola, causes rotting and blackleg in potato. To control this pathogen using bacteriophage therapy, we isolated and characterized two closely related and specific bacteriophages, vB_DsoM_LIMEstone1 and vB_DsoM_LIMEstone2. The LIMEstone phages have a T4-related genome organization and share DNA similarity with Salmonella phage ViI. Microbiological and molecular characterization of the phages deemed them suitable and promising for use in phage therapy. The phages reduced disease incidence and seve