姜黄素对9月龄AD小鼠学习记忆海马超微结构的影响.doc

研究报告 姜黄素对9月龄的影响 樊惠1,2，1,2，1,2，1,2，任映1,2，孙海芸1,2，杨金铎1,2，王蓬文1,2 国家中医药管理局三级实验室北京中医药大学东直门医院中药药理学实验室北京 100700；2.，北京 100700 【摘要目的 应用Morris水迷宫，AD模型APPswe/PS1dE9双转基因小鼠定位航行及空间搜索等学习和记忆保持能力，评价姜黄素对APPswe/PS1dE9双转基因小鼠认知功能的影响。方法 将月龄的APPswe/PS1dE9双转基因小鼠随机分为模型组、罗格列酮组(10 mgkg?d)、姜黄素(400 mg/kg?d)、中(200 mgkg?d)、剂量组(100 mgkg?d)，每组只；并以同月龄遗传背景相同的C57/BL6J小鼠作为对照组。每天灌胃给药1次，连续灌胃个月。应用Morris水迷宫。结果 姜黄素对APPswe/PS1dE9双转基因小鼠空间学习记忆能力障碍有改善作用，尤以姜黄素剂量效果比较明显结论 姜黄素能改APPswe/PS1dE9双转基因小鼠的。 关键词姜黄素APPswe/PS1dE9双转基因小鼠Morris 水迷宫 【中图分类号】R33【文献标识码】A【文章编号】1671-7856（201）-0000-00 Doi:10.3969/j.issn.1671-7856.2013.006.000 Effect of curcumin on the ethology of APPswe/PS1dE9 transgenic mice FAN hui1,2,WEI Peng1,2,CHEN Xiao-pei1,2,FENG Hui-li1,2,REN Ying1,2,SUN Hai-yun1,2,YANG Jin-duo1,2,WANG Peng-wen1,2 （1. Key Laboratory of Pharmacology of Dongzhimen Hospital, State Administration of Traditional Chinese Medicine, 2. Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China） Abstract】 Objective To evaluate the effect of curcumin on cognitive function of APPswe/PS1dE9 double transgenic mice. Methods 3-month old APPswe/PS1dE9 dtg mice were randomly divided into model group, rosiglitazone group and curcumin high-, medium- and low-dose groups (n = 6), with age-matched C57BL/6J mice of the same genetic background as normal control group. Medication was given once a day by intragastric gavage for 6 months. Morris water maze was used to test the spatial learning and memory ability of APPswe/PS1dE9 double transgenic mice. Results Curcumin showed improvement effect on the spatial learning and memory disabilities of APPswe/PS1dE9 double transgenic mice, especially in the curcumin high-dose and medium-dose groups. Conclusion Curcumin can improve learning and memory ability of APPswe/PS1dE9 double transgenic mice. 【Key words】Curcumin; APPswe/PS1dE9 double tr