MFAP3L在胃癌中的表达及与PI3K_AKT信号转导通路的关系.pdf

·806 · 肿瘤防治研究 20 10 年第 37 卷第 7 期 DO I :10 . 397 1/ j . i ssn .. 20 10 . 07 . 0 19 M FA P3L 在胃癌中的表达及与 P I3 K/ A KT 信号转导通路的关系 张庆英 ,成文翔 ,周太梅 ,易德青 M FA P3L Expression in Gastric Cancer and Potential Role in PI3 K/ A KT Pathway ZHAN G Qingying , C H EN G Wenxiang , ZHOU Taimei , YI Deqing D ep a rtment of P reventi ve M e d ici ne , S hantou Uni ve rs i ty M e d ical Col leg e , S hantou 5 1504 1 , Chi na Abstract : Objective 　To investigat e t he M FA P3L exp ression in gast ric cancer ( GC) and it s pot ential role in t he PI3 K/ Akt p at hway . Methods 　R TPCR and microarray IHC were p erformed to det ect t he mRN A and p rot ein exp ression of M FA P3L , PI K3 CA , A KT2 and Glaectin3 in p rimary stomach t umor s. Bioinformatics met ho ds were u sed to analyze t he p rot ein st ruct ure of M FA P3L . Results 　 M FA P3L , Glaectin3 and PI K3 CA p rot ein were highly exp ressed in p rimary stomach t umor (70 . 8 % , 17/ 24 , P = 0 . 022 ; 16/ 25 , 64 . 0 % , P = 0 . 0 17 ; 14/ 25 , 56 . 0 % , P = 0 . 04 5) . P IK3CA gene mRN A wa s highly exp ressed in GC (65 . 5 % 19/ 29 , P = 0 . 00 1) a s well a s A KT2 mRN A (56 . 0 % , 14/ 25 , P = 0 . 00 1 ) . Glaectin3 p rot ein coexp ressed wit h Glaectin3 and P IK3CA p rot ein . A dditionally , M FA P3L cont ain s multiple p ho sp horylation sit es and a con servative t ran smembrane domain . Conclu sion 　Thi s st udy demon st rat ed t hat high exp ression of M FA P3L may be relevant to stomach t umori genesi s and get involved wit h PI3 K/ Akt p at hway to facilit at e t umor inva sion and met a st a si s. Key words : M FA P3L ; PI K3CA ; A KT2 ; Galectin3 ; Gast ric cancer 摘 　要 : 目的　了解微纤维关联样蛋 白3 ( M FA P3L ) 基因在 胃癌组织中的表达情况并分析其与 PI3 K/ A KT 信号转导通路相关基因间的关系 。方法 　采用半定量 R TPCR 和组织微阵列免疫组化 α(