生血增白汤在小鼠骨髓移植后造血重建中的作用-国际中医中药杂志.pdf

·400 · 国际中医中药杂志2011年5月第33卷第5期 Int J Trad Chin Med, May 2011, Vol. 33, No.5 ·实验研究 · 生血增白汤在小鼠骨髓移植后 造血重建中的作用 张栋 孙金芳 孙静 【摘要】 目的 探讨生血增白汤对骨髓移植后小鼠骨髓中细胞间黏附分子(ICAM-1)、干细胞因子 (SCF)表达水平的影响。方法 将实验动物采用随机数字表法分为 5 组各 30 只：正常组，未做任何处理； 对照组、G-CSF 组、生血组、生血＋G-CSF 组，建立小鼠骨髓移植模型，生血组及生血＋G-CSF 组胃饲 生血增白汤浓缩煎剂。骨髓移植后的第 9、14、24 天，采用免疫组化法检测 ICAM-1 、SCF 表达水平，进 行外周血细胞和骨髓单核细胞计数，并做骨髓组织学观察。结果 骨髓移植后第 9、14、24 天生血组 ICAM-1 （34.21 ±5.54、14.42±3.36、8.59 ±4.03 ）及 SCF （50.40 ±5.03、45.61 ±3.85、43.22 ±4.71 ）的 表达水平、外周血白细胞（2.66 ±0.89 ）、血红蛋白（149.67±21.64 ）、骨髓单核细胞计数（2.81 ±0.20 ） 及骨髓细胞增生程度均高于其他各组（P ＜0.05 或 P ＜0.01 ）。结论 生血增白汤可促进骨髓移植后骨髓中 ICAM-1 及 SCF 的表达。 【关键词】 血虚；骨髓移植；造血重建 Study on the effects of Shengxue-zengbai Decoction on the hematopoietic reconstitution in * bone marrow transplanted mice and its molecular mechanism ZHANG Dong , SUN Jin-fang, SUN Jing. *Qingdao Haici Hosp, Qingdao 266033, China Corresponding author：SUN Jing, Email ：sunsunjingjing@163.com 【Abstract 】 Objective To investigate the effect of Shengxue-zengbai Decoction on the expression of ICAM-1 and SCF in bone marrow tissue and on the mechanism of hematopoietic reconstitution in bone marrow transplantation (BMT). Methods The experimental animals were randomly divided into 5 groups: normal group received no treatment, BMT control group, G-CSF group, Shengxue group, and Shengxue ＋G-CSF group. BMT mouse models were established. Shengxue group, Shengxue ＋G-CSF group were given Shengxuezengbai Decoction. On days 9, 14, 24 after BMT, mice were killed and peripheral blood cells, bone marrow nucleated cells were detected. Histologic