多壁碳纳米管固定化生物酶修饰电极检测杂色曲霉素的初步研究.pdf

20 4 Vol . 20 No. 4 2004 7 ChineseJournal of Biotechnology July 2004 1 1 1* 1 2 1 姚冬生 文圣梅 刘大岭 谢春芳 白 燕 冉艳红 ( 1 , 2 , 5 10632) ( ST) , ST TLCHPLCELISA PCR ST , ADTZ Au , ST CV DPV , : ST - 600mV , 832 10- 5 ~ 6656 10- 5mgmL, 832 10- 5mgmL, 10s, , , , Q503 A 2004) ( Ster gmatocyst n, ST ) THERMO , USA) , (TECNAI 10, Ph l ps, 1954 ( Asperg llusvers color) Heland) , ( BAS100, BAS , USA) , [ 1] , : AgAgCl , Pt ( sterigmatocystin ( ST ) O ( BAS100 ) ; : Au ( methylster gmatocyst n ( OMST ) AFB1) [2] , ) , pH ( METTLER TOLEDO 320S, , ( Aflatox n, AFT) ) , ( Mult wall carbon nanotube, , , MWNT) , ( Sorvall [ 3] RC5B, Dupont , USA) , ( Stger gmatocys : t n, ST ) S gma , (TLC) , ( ELISA) , , ( HPLC) , PCR [ 4- 12] [15, 16] 12 MWNT ( : = 1!3) 9 ~ 10h, 10 000g 15m n , , (Aflatox ndetox nf zyme, ADTZ) AFT , 10 000g, 15m n , 2 , ADTZ ST [ 13, 14] 1mg , MWNT 10mL ( DMF) , , , 01mgmL 13 [ 17]