YAP在菲小细胞肺癌增殖侵袭中的作用研究的开题报告.pdf
YAP在菲小细胞肺癌增殖侵袭中的作用研究的开题
报告
BackgroundandRationale:
Lungcancerisoneoftheleadingcausesofcancermortality
worldwide.Smallcelllungcancer(SCLC)accountsforabout10-15%
ofalllungcancersandischaracterizedbyitsrapidgrowthand
metastasis,makingitmoredifficulttotreat.Theidentificationofnew
moleculartargetsforthetreatmentofSCLCremainsacrucialareaof
research.TheYAPproteinhasbeenshowntoplayaroleincell
proliferation,migration,andinvasioninvarioustypesofcancers.
However,itsroleinSCLCremainspoorlyunderstood.
Objectives:
ToinvestigatetheroleofYAPinSCLCproliferation,invasion,and
metastasis.Specifically,weaimto:
1.CharacterizetheexpressionofYAPinSCLCcelllinesandpatient
samples.
2.DeterminetheeffectsofYAPknockdownoroverexpressionon
SCLCcellproliferationandinvasion.
3.InvestigatethemechanismsbywhichYAPpromotesSCLC
growthandmetastasis.
Methods:
1.Expressionanalysis:WewillanalyzetheexpressionofYAPin
SCLCpatientsamplesandcelllinesbywesternblottingand
immunohistochemistry.
2.Knockdown/overexpression:Wewilluselentiviral-mediated
knockdownoroverexpressionofYAPinSCLCcellsandassessthe
effectsoncellgrowthandmigrationbycellproliferationassays,wound
healingassays,andtranswellinvasionassays.
3.Mechanisticstudies:Wewillinvestigatethedownstream
signalingpathwaysandtargetgenesofYAPinSCLCcellsusingRNA
sequencingandqPCR.
Expectedoutcomes:
ThestudyisexpectedtoprovideinsightsintotheroleofYAPin
SCLCproliferation,invasion,andmetastasis.WeanticipatethatYAP
knockdownorinhibitionwillleadtodecreasedSCLCcellproliferation
andinvasion.Weexpecttoidentifypotentialdownstreammediatorsof
YAPsignalinginSCLCcellsthatcouldserveastherapeutictar