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YAP在菲小细胞肺癌增殖侵袭中的作用研究的开题报告.pdf

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YAP在菲小细胞肺癌增殖侵袭中的作用研究的开题

报告

BackgroundandRationale:

Lungcancerisoneoftheleadingcausesofcancermortality

worldwide.Smallcelllungcancer(SCLC)accountsforabout10-15%

ofalllungcancersandischaracterizedbyitsrapidgrowthand

metastasis,makingitmoredifficulttotreat.Theidentificationofnew

moleculartargetsforthetreatmentofSCLCremainsacrucialareaof

research.TheYAPproteinhasbeenshowntoplayaroleincell

proliferation,migration,andinvasioninvarioustypesofcancers.

However,itsroleinSCLCremainspoorlyunderstood.

Objectives:

ToinvestigatetheroleofYAPinSCLCproliferation,invasion,and

metastasis.Specifically,weaimto:

1.CharacterizetheexpressionofYAPinSCLCcelllinesandpatient

samples.

2.DeterminetheeffectsofYAPknockdownoroverexpressionon

SCLCcellproliferationandinvasion.

3.InvestigatethemechanismsbywhichYAPpromotesSCLC

growthandmetastasis.

Methods:

1.Expressionanalysis:WewillanalyzetheexpressionofYAPin

SCLCpatientsamplesandcelllinesbywesternblottingand

immunohistochemistry.

2.Knockdown/overexpression:Wewilluselentiviral-mediated

knockdownoroverexpressionofYAPinSCLCcellsandassessthe

effectsoncellgrowthandmigrationbycellproliferationassays,wound

healingassays,andtranswellinvasionassays.

3.Mechanisticstudies:Wewillinvestigatethedownstream

signalingpathwaysandtargetgenesofYAPinSCLCcellsusingRNA

sequencingandqPCR.

Expectedoutcomes:

ThestudyisexpectedtoprovideinsightsintotheroleofYAPin

SCLCproliferation,invasion,andmetastasis.WeanticipatethatYAP

knockdownorinhibitionwillleadtodecreasedSCLCcellproliferation

andinvasion.Weexpecttoidentifypotentialdownstreammediatorsof

YAPsignalinginSCLCcellsthatcouldserveastherapeutictar

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