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一个水稻卷叶基因rl(t)的精细定位.pdf

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中国水稻科学(ChinJRiceSci),2011,25(5):455—460 http:|} .ricesci.cn DOI:l0.3969/j.issn.100卜7216.2011.05.001 455 一 个水稻卷叶基因rf(t)的精细定位 潘存红 李 磊 陈宗祥 薛 芗 张亚芳 左示敏 戴正元 潘学彪 马玉银 。, (t扬州大学 江苏省作物遗传生理重点实验室 /植物功能基 因组学教育部重点实验室 ,江苏 扬州 225009; 江苏里下河地区农业科学研究N / 国家水稻改 良中心南京分 中心 ,江苏 扬州 225007;扬州职业大学 生化工程系,江苏 扬州 225009; 共 同第一作者 ;通讯联系人 ,E—mail: shuidao@yzu.edu.en;mayuyinl26@126.corn) FineM appingofaRolledLeafGenerl(t)inRice PANCun-hong’’ ,LtLei’ ,CHENZong—xiang ,XUEXiang ,ZHANGYa—fang ,ZuoShi—rain ,DAIZheng-yuan , PANXue—biao .MA Yu—yin ’’ (KeyLaboratoryofCropGeneticsandPhysiologyofJiangsuProvince/KeyLaboratoryofPlantFunctionalGenomicsofMinistryofEd— ucation,YangzhouUniversity,Yangzhou225009,China; LixiaheAgriculturalResearchInstituteoJJiangsuProvinee/NanjingSubcenter ofNationalRiceImprovementCenter,Yangzhou225007,China;BiochemicalEngineeringDepa~Zment,YangzhouPolytechnicCollege, Yangzhou225009,China; Theseauthorscontributeequallytothispaper; Correspondingauthors,E-mail:shuidao@yzu.edu.cn;mayuy— inl26国 126.corn) PAN Cunhong,IA Lei,CHEN Zongxiang,eta1.Finemappingofarolledleafgenerl㈨ inrice.ChinJRiceSci,2011,25(5): 455—460. Abstract:M oderatelyrolledleafisoneofthemostimportantmorphol0gica1charactersin idealplantarchitecturebreeding in rice.Theheterozygousrl(t)genecouldproducemoderatelyrolledleafandincreasericeyield,presentingahighbreedingpoten— tia1.ByusingBC5F and BC5Fdpopulationsderivedfrom arolledleafZhenshan97B(carryingrl()gene)andaflat—leafvariety Qimiaoxiang(recurrentparent),andatotalof15polymorphicmarkers,therl(tgenewasultimatelymappedintoaregionof 11kbflankedbythemarkersP95053andP113.6onchromosome2.Thegeneticdistanceofthisregionisabout0.04cM.On— lyonepredicatedORF (openreadingframe)/genewasfoundinthisregion,whichencodesatranscriptionfactoroftheho— meoboxfamilyHD-GI2(alsoknownasHD-ZIPⅣ).Theresultslaidafoundationforfurthercloningofrlf1【and
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