苦参碱抑制人雄激素非依赖性PC3细胞增殖和you导凋亡的研究.doc

　　苦参碱抑制人雄激素非依赖性PC3细胞增殖和诱导凋亡的研究 作者：张鹏，王子明，纪宗正，种铁，黄辰，高彩霞 【摘要】 目的 研究苦参碱对人雄激素非依赖性前列腺癌细胞株PC3增殖、细胞周期及细胞凋亡的影响。方法 不同浓度苦参碱作用PC3细胞后，应用MTT法观察苦参碱对PC3细胞增殖的影响，流式细胞仪研究苦参碱对PC3细胞周期的影响，TUNEL法、Annexin V/PI双染分析苦参碱对PC3细胞凋亡的影响。结果 苦参碱对PC3细胞的生长具有抑制作用，与对照组相比有统计学差异(Plt;0.05)。苦参碱对PC3细胞的增殖抑制作用呈现时间及剂量依赖性关系。苦参碱的最佳药物浓度为1.0g/L，最佳作用时间为48h。流式细胞仪分析显示不同浓度苦参碱均可使PC3细胞阻滞于G0/G1期，随着苦参碱浓度的增加，G0/G1期细胞所占的比例增加，与对照组相比有统计学差异(Plt;0.01)。TUNEL法、Annexin V/PI分析显示不同浓度苦参碱均可诱导PC3细胞凋亡，其早期凋亡率及晚期凋亡率随着苦参碱浓度的增加而升高，呈现剂量依赖性关系(Plt;0.05，Plt;0.01)。结论 苦参碱在体外可抑制PC3细胞的增殖，具有时间和浓度依赖关系。抑制增殖和细胞周期阻滞与G0/G1期相关。苦参碱可诱导PC3细胞凋亡，与苦参碱的作用浓度及时间有关。 【关键词】 前列腺癌;苦参碱;细胞凋亡 　　ABSTRACT: Objective To investigate the effects of matrine on the proliferation, cell cycle, apoptosis of androgen independent prostate cancer (AIPC) cell line PC3 in vitro. Methods PC3 cells were treated by different concentrations of matrine. The inhibitory effect of matrine on the proliferation of PC3 cells was observed by MTT method. The cell cycle of PC3 cells treated by matrine was observed by flow cytometric assay. Apoptosis of PC3 cells induced by matrine was determined with TUNEL and Annexin V/PI staining assay. Results MTT test showed that PC3 cells treated by matrine had an inhibitory effect, which was significantly different from that of controls (Plt;0.05). Matrine markedly inhibited cell proliferation of PC3 in dose and timedependent manners. The optimal dose of matrine which inhibited PC3 cells was 1.0g/L and the best time of inhibition was at 48h. Flow cytometric assay showed that the PC3 cells treated by different doses of matrine were arrested in cell cycle progression at G0/G1 phase. The cell ratio at G0/G1 phase increased with the increase of matrine dose, which was significantly different from that of controls (Plt;0.01). TUNEL and Annexin V/PI staining assay showed that matrine could obviously induce the apoptosis of PC3 cells. The ratio of early and late apoptosis of PC3 cells treatedby matrine was increased in a dosedependent manner (Plt;0.05,